Technique to minimize phenolics in walnut in vitro culture initiation
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https://doi.org/10.5958/0974-0112.2017.000057.3Keywords:
Walnut, phenolic content, antioxidants, in vitro culture, ascorbic acid.Issue
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Abstract
Total phenol content of the walnut genotypes was determined to explore the relationship between total phenols and regeneration response for different walnut genotypes. Walnut leaves from ten genotypes (CITHWalnut- I, CITH-Walnut-II, BBW-8, CITH-Walnut-IV, BP-3, SP-1, LG-11, Hamdan, Suleiman, and Opex Culchery) were used for extraction of total phenols through modified Folin-Ciocalteu method. Total leaf phenolic content ranges from 140 μg g-1 (WGB-1) to 285 μg g-1 (BBW-8). Phenolic interactions expressed as darkening of the explants lead to death. Among different antioxidants used ascorbic acid @ 350 mg/l was found best with almost no phenol exudation in the medium and shoot initiation occurred after 8 days of inoculation. The number of shoots was highest (10), followed by citric acid used @ 350 mg/l showing low degree of exudation where shoot initiation was noted in 15 days and with 15.0 shhots per explant.
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