RNA isolation from high polyphenol containing tissues of pomegranate

Abstract

Three methods of RNA isolation were evaluated based on quality and quantity of the RNA isolated. The methods used for RNA isolation were CTAB-LiCl method, Direct-zol™ RNA MiniPrep (ZymoResearch), and TRIzol® reagent (Life Tech.). RNA quality was assessed using spectrophotometric methods, agarose gel electrophoresis and Agilent 2100 Bioanalyzer. RNA isolation using modified CTAB-LiCl was found effective in extraction of sufficient quantity and high quality RNA from new leaves (617 μg/g FW), seedlings (636 μg/g FW) and flower buds (603 μg/g FW) having RNA integrity number (RIN) values of 8.5 each.