Rapid regeneration and molecular assessment of genetic stability using RAPD markers in stevia
Downloads
Published
Keywords:
Genetic fidelity, Stevia, micropropagation, PEG, RAPD analysis.Issue
Section
License
Copyright (c) 2016 Indian Journal of Horticulture

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Abstract
The present study was carried out to monitor somaclonal variations in Stevia rebaudiana using random amplified polymorphic DNA (RAPD) markers. Nodal and shoot tips were used as explants. Maximum shoot induction (98.0 ± 2.00%) in (4.7 ± 0.20) days was reported in nodal explants and (74.0 ± 2.45%) in (7.1 ± 0.34) days in shoot tip explants on MS medium supplemented with BAP 2.0 mg/l (EM4). The regenerated shoots were cultured on multiplication media and maximum (40.5 ± 0.26) shoots were observed on MS medium fortified with 0.3 mg/l BAP + 0.3 mg/l KIN + 0.1 mg/l NAA + 15 mg/l PEG on 30th day of culturing. Maximum 100% rooting was reported on 1/2 MS medium supplemented with 0.5 mg/l NAA with (21.2 ± 0.3) roots/shoot in 7.4 ± 0.26 days. Rooted shootlets were separated individually and hardened in green house. The hardened plants of Stevia were screened for genetic stability using RAPD primers. Total twenty four RAPD primers were used which produced 82 distinct and scorable bands, with an average of 3.4 bands per primer and the amplification products range was from 100-1100 bp. The number of scorable bands for RAPD primer varied from 1 to 7. RAPD profiles from micropropagated plants were monomorphic and similar to mother plants, confirming their genetic stability. The results corroborate the fact that in vitro multiplication is the safest mode for production of true-to-the-type plants.
How to Cite
Downloads
Similar Articles
- Chitra Devi Pandey, Sushil Pandey, Hot water treatment for improving germination in Melochia corchorifolia , Indian Journal of Horticulture: Vol. 72 No. 04 (2015): Indian Journal of Horticulture
- Jagdev Sharma, Ajay K. Upadhyay, Dyanesh Bande, Sarika D. Patil, Studies on black leaf symptom development and its impact on nutrient profile and fruitfulness in Thompson Seedless grapevines grafted on Dogridge rootstock , Indian Journal of Horticulture: Vol. 67 No. 02 (2010): Indian Journal of Horticulture
- Shilpa Devi, A. D. Munshi, S. S. Dey, T. K. Behera, Chellapilla Bhardwaj, Kishor Gaikwad, Arpan Bhowmik, Arvind Nagar, Generation mean analysis of earliness and fruit yield related traits in Cucumber , Indian Journal of Horticulture: Vol. 80 No. 1 (2023): Indian Journal of Horticulture
- Arindam Das, A. V. V. Koundinya, Manas Kumar Pandit, Santanu Layek, Saheb Pal, Interrelationship and multivariate analysis of floral and fruit attributes in brinjal , Indian Journal of Horticulture: Vol. 75 No. 04 (2018): Indian Journal of Horticulture
- P.L. Saran, A.K. Godara, S.K. Sehrawat, Morphological variability among low chilling peach genotypes under Dehradun conditions , Indian Journal of Horticulture: Vol. 67 No. 01 (2010): Indian Journal of Horticulture
- K. Sethi, K. Pradhan, K.C. Mohapatra, P. Tripat, P.L. Saroj, Stability analysis for nut yield and component traits in cashew , Indian Journal of Horticulture: Vol. 74 No. 01 (2017): Indian Journal of Horticulture
- R.K. Sharma, P.K. Choudhary, Arun Agarwal, Efficient microspore embryogenesis for the development of doubled haploid plants in broccoli , Indian Journal of Horticulture: Vol. 79 No. 02 (2022): Indian Journal of Horticulture
- R.A. Marathe, K. Dhinesh Babu, Determination of sampling period and leaf position for critical nutrient analysis in pomegranate cv. Bhagwa , Indian Journal of Horticulture: Vol. 72 No. 04 (2015): Indian Journal of Horticulture
- R. Sharma, M. Modgil, P. Sharma, U. Saini, Agrobacterium-mediated transfer of chitinase gene in apple (Malus x domestica Borkh.) rootstock MM106 , Indian Journal of Horticulture: Vol. 69 No. 01 (2012): Indian Journal of Horticulture
- A.L. Meitei, R.K. Pamarthi, Raj Kumar, N.T. Bhutia, D. Rai, P. Kiran Babu, A.K. Singh, R. Gazmer, D.R. Singh, Dendrobium nobile orchid in traditional medicine - A phytochemical analysis , Indian Journal of Horticulture: Vol. 76 No. 03 (2019): Indian Journal of Horticulture
<< < 29 30 31 32 33 34 35 36 37 38 > >>
You may also start an advanced similarity search for this article.