Identification of self-incompatibility in early cauliflower using fluorescence microscopy
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Abstract
In cauliflower, sporophytic incompatibility is the genetic mechanism which ensures cross pollination. F1 hybrid culture has assumed much more importance; of late.The Production of hybrids making use of incompatibility mechanism requires the identification of self incompatible lines. The usual procedure of ascertaining the pollen–stigma compatibility relationships from the seed set data is not only laborious but also a time consuming process. Alternatively, fluorescence microscopy can be advantageously used, as the results are known within eight to ten days (Vidyasagar and Chatterjee, 8). The present study,was, therefore, undertaken to study the efficiency of seed set method and fluorescence microscopy methods to identify self-incompatible lines in early cauliflower.
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