Preservation of a GI-tagged banana cultivar: Insights from tissue culture and DNA barcoding of Nanjangud Rasabale

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Published

2025-09-30

DOI:

https://doi.org/10.58993/ijh/2025.82.3.7

Keywords:

Genome sequencing, DNA barcoding, tissue culture, ITS2, biodiversity conservation
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Issue

Vol. 82 No. 03 (2025): Indian Journal of Horticulture

Section

Research Articles

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Copyright (c) 2025 Ajay Bhat, Subrahmanya Hegde, Charukesi R, Thotegowdanapalya C Mohan

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This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Authors

  • Ajay Bhat Department of Biotechnology & Bioinformatics, JSS Academy of Higher Education & Research, Mysuru, Karnataka, India. 570015.
  • Subrahmanya Hegde
  • Charukesi R
  • Thotegowdanapalya C Mohan

Abstract

Nanjanagudu Rasabale (NRB) is a Geographical Indication (GI)-tagged banana cultivar from Karnataka, known for its unique aroma and taste. The cultivar faced a serious threat of extinction due to a major outbreak of Panama disease. This study aimed to conserve and verify the genetic identity of NRB using tissue culture and DNA barcoding techniques. Shoot tip culture was used to produce disease-free saplings, and DNA barcoding was performed using the ITS2 region as a marker. The tissue culture protocol showed a high rate of micropropagation, enabling large-scale production of healthy plantlets suitable for field planting. However, DNA barcoding alone was not sufficient to distinguish NRB from closely related cultivars, highlighting the need for morpho-molecular analysis and whole genome sequencing for accurate identification. To support conservation, the disease-free saplings were distributed to farmers across different districts of Karnataka, and their contact information has been recorded for follow-up.

How to Cite

Bhat, A., Hegde, S., R, C., & C Mohan, T. (2025). Preservation of a GI-tagged banana cultivar: Insights from tissue culture and DNA barcoding of Nanjangud Rasabale. Indian Journal of Horticulture, 82(03), 297–302. https://doi.org/10.58993/ijh/2025.82.3.7

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References

1. Ahmad, K., Quamari, M. A., Ahmad, H. and Hafiz, K. A. 2023. Phytochemical profile and pharmacological activities of karanjawa (Caesalpinia bonducella L.): An important botanical origin drug of unani system of medicine. Rguhs J. Ayush Sci. 10(1).

2. Attia, M. M., Al Sayed, A. A. K. A., Tayeh, B. A. and Shawky, S. M. 2022. Banana agriculture waste as eco-friendly material in fibre-reinforced concrete: An experimental study. Adv. Concr. Constr. 14, 355-368.

3. Babu, P. 2019. An efficient protocol for in vitro regeneration of banana var. nanjangudu rasabale (Musa spp. AAB). Int. J. Curr. Microbiol. App. Sci. 8(6), 3392-3402.

4. Bhat, A. R., Hegde, S., Kammar, S. S., Muthamma, M. B., Mudgal, G., Charukesi, R. and Mohan, T. C. 2024. Curating and validating universally applicable primers for efficient ITS2- based DNA barcoding across plant taxa. Int. J. Health Allied Sci. 13(1), 4.

5. Chand, K., Singh, S. K., Meena, R., Nain, Y. and Choudhary, K. 2021. Roving survey of panama wilt disease (Fusarium oxysporum f. sp. cubense) of banana in Bihar. J. Exp. Agric. Int. 43(12), 1-7.

6. Guranna, P., Hegde, R. and Govindaswamy, B. 2018. In vitro regeneration of banana cv. Nanjanagud Rasabale (AAB) by shoot tip culture. Biotechnol. J. Int. 20(4), 1-9.

7. Hřibová, E., Čížková, J., Christelová, P., Taudien, S., de Langhe, E. and Doležel, J. 2011. The ITS1-5.8 S-ITS2 sequence region in the Musaceae: structure, diversity and use in molecular phylogeny. Plos one, 6(3), e17863.

8. Lamb, J. M. and Naidoo, P. 2016. Largely invariant trnL-F/nrITS sequences and ISSRs do not resolve morphospecies of Warburgia (Canellaceae). Phytotaxa, 270(4), 252.

9. Li DeZhu, L. D., Gao LianMing, G. L., Li HongTao, L. H., Wang Hong, W. H., Ge XueJun, G. X., Liu Jian Quan, L. J. and Duan GuangWen, D. G. 2011. Comparative analysis of a large dataset indicates that internal transcribed spacer (ITS) should be incorporated into the core barcode for seed plants.

10. Li, X., Yang, Y., Henry, R. J., Rossetto, M., Wang, Y. and Chen, S. 2015. Plant DNA barcoding: from gene to genome. Biol. Rev. 90(1), 157-166.

11. Raghavendra, H. R., Hiremath, V., Tejukumar, B. K. and Udaya, T. V. 2022. Geographical indications of fruit crops. Indian Hort. 66(6).

12. Sokołowska, J., Fuchs, H. and Celiński, K. 2022. Assessment of ITS2 region relevance for taxa discrimination and phylogenetic inference among Pinaceae. Plants. 11(8), 1078.

13. Suman, S. 2017. Plant tissue culture: A promising tool of quality material production with special reference to micropropagation of banana. Biochem. Cell. Arch. 17(1).

14. Vasconcelos, S., Nunes, G. L., Dias, M. C., Lorena, J., Oliveira, R. R., Lima, T. G. and Oliveira, G. 2021. Unraveling the plant diversity of the Amazonian canga through DNA barcoding. Ecol. Evol. 11(19), 13348-13362.

15. Yao, H., Song, J., Liu, C., Luo, K., Han, J., Li, Y. and Chen, S. 2010. Use of ITS2 region as the universal DNA barcode for plants and animals. PloS one, 5(10), e13102.

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