An efficient protocol for shoot organogenesis and plant regeneration in jackfruit

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Published

2022-12-05

Keywords:

Artocarpus heterophyllus, micropropagation, BAP, GA3, plant growth regulators
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Issue

Vol. 79 No. 3 (2022): Indian Journal of Horticulture

Section

Research Articles

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Copyright (c) 2022 Indian Journal of Horticulture

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This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Authors

  • Ratna Rai Department of Horticulture, G.B. Pant University of Agriculture & Technology, Pantnagar– 263145, U.S. Nagar, Uttarakhand, India
  • Divyangana Department of Horticulture, G.B. Pant University of Agriculture & Technology, Pantnagar– 263145, U.S. Nagar, Uttarakhand, India
  • Ranjan Srivastava Department of Horticulture, G.B. Pant University of Agriculture & Technology, Pantnagar– 263145, U.S. Nagar, Uttarakhand, India
  • Rajesh Kumar Department of Horticulture, G.B. Pant University of Agriculture & Technology, Pantnagar– 263145, U.S. Nagar, Uttarakhand, India
  • V.P. Singh Department of Horticulture, G.B. Pant University of Agriculture & Technology, Pantnagar– 263145, U.S. Nagar, Uttarakhand, India
  • Pratibha Department of Horticulture, G.B. Pant University of Agriculture & Technology, Pantnagar– 263145, U.S. Nagar, Uttarakhand, India

Abstract

The present investigation was carried out for the rapid clonal mass multiplication of disease free plants of jackfruit cv. Pant Garima. The nodal explants were collected in winter season proved to be the best with regard to maximum survival of explants. The effect of sterilants (HgCl2 and ethanol) in various combinations was also assessed on survival percentage of explants. The MS medium was supplemented with five concentrations of BAP (1.0, 2.0, 3.0, 4.0 and 5.0 mgL-1) along with control (0.0mgL-1) and their effects on shootlets regeneration, and length of shootlets were evaluated. Maximum shoot proliferation was obtained in the medium supplemented with 4.0 mgL-1 BAP. Further, GA3 @ 1.0 mgL-1 added to MS medium supplemented with 4.0 mgL-1 BAP gave the best results for enhancing the length of the shootlets. For rooting, half strength of MS liquid medium fortified with 2.0 mgL1 IBA gave maximum rooting.

How to Cite

Rai, R., Divyangana, Srivastava, R., Kumar, R., Singh, V., & Pratibha. (2022). An efficient protocol for shoot organogenesis and plant regeneration in jackfruit. Indian Journal of Horticulture, 79(3). Retrieved from https://journal.iahs.org.in/index.php/ijh/article/view/483

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