Genetic diversity in cassava based on agronomical, physiological and EST-microsatellite markers under moisture stress conditions
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https://doi.org/10.58993/ijh/2023.80.4.3Keywords:
Manihot esculenta, drought, agro-physiological, EST-Microsatellites polymorphism, genetic diversityIssue
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The genetic diversity of 23 cassava accessions was studied using agro-physiological and ExpressedAbstract
Sequence Tags (EST)-Microsatellite markers. The genotypes were classified into six clusters based on
agronomical and physiological parameters. Cluster-2 had the highest number of genotypes (10), while clusters-5
and 6 had single accession, respectively. The high-yielding and highly drought-tolerant genotype 8S501
stood alone in cluster 5. It had high cluster mean values for the majority of the traits. The genotypes present
in cluster-4 & 5 and cluster-1 & 2 were divergent and hybridisation between these two groups may result in
superior genotypes for drought tolerance. The EST-Microsatellites obtained from the drought transcriptome
of cassava showed that the mean number of alleles per locus was 1.81, with an average size of 217.7 bp. The
locus, MeESSR47 (Thylakoid membrane phosphoprotein 14 kDa, chloroplast precursor, putative), had more
heterozygosity (0.387) and Shannon’s Index (0.575) among all the loci. Cumulatively, these results showed
low polymorphism in the EST regions of cassava DNA. The dendrogram showed 4 clusters based on EST
microsatellite diversity. The microsatellite-based cluster classification of genotypes did not follow the diversity
based on agronomical traits under moisture stress conditions. A weak correlation revealed by the Mantel test
also indicated no relation between agronomical and molecular diversity.
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