Detection of apple mosaic and chlorotic leaf spot viruses by DAS-ELISA from farmers orchards of Kashmir valley

Abstract

The prevalence of Apple mosaic virus (ApMV) and Chlorotic leaf spot virus (ACLSV) was investigated in the major apple growing areas of Kashmir valley. Leaf samples collected from fifty two orchards showed virus like visible symptoms. All samples were analyzed to detect the viruses through double antibody sandwich- Enzyme linked immuno sorbent assay (DAS-ELISA). Samples of about 69% of orchard showed infection for ApMV and 73% for ACLSV. Maximum incidence of both the viruses were observed in apple varieties ‘Mollies Delicious’, ‘Red Delicious’ and ‘Golden Delicious’, ‘Starkrimson’, ‘Maharaji’ and in variety ‘Jonica’, ‘Chamoora’, ‘Hazratbali’, ‘Red Chief’, ‘American Apirouge’ and ‘Ambri’, virus infection was absent. The present study indicated the occurrence and distribution of ApMV and ACLSV in Kashmir valley.