In vitro propagation of a self-incompatible cabbage line ‘Sel. 5’
Downloads
Published
Keywords:
Cabbage, self-incompatibility, micropropagation, growth hormones.Issue
Section
License
Copyright (c) 2013 Indian Journal of Horticulture
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Abstract
A viable protocol was developed for in vitro maintenance and multiplication of cabbage SI line ‘Selection-5’ with very strong S-allele interaction. Different types of explants, viz., apical bud, axillary bud and basal shoot sprout were tested for their in vitro regeneration ability. The morphogenetic potential varied among the explants. Apical bud proved to be the most potent explant for initial culture establishment followed by axillary bud. Murashige and Skoog (1962) medium supplemented with 2 mg l-1 BA, 0.5 mg l-1 NAA and 0.1 mg l-1 GA3 was optimum for culture establishment. The maximum in vitro shoot proliferation (5.89 ± 0.38) was obtained on MS medium supplemented with 5 mg l-1 KIN + 0.1 mg l-1 NAA + 0.1 mg l-1 GA3. The proliferation rate was significantly influenced by type and concentration of cytokinins with kinetin being more effective than BA. Half-strength MS medium supplemented with 1.0 mg l-1 IBA was most effective for rooting. The tissue cultured plants were successfully hardened and transferred to field conditions with a survival rate of 76.67 per cent.
How to Cite
Downloads
Similar Articles
- M.S. Saraswathi, S. Praveena, S. Uma, R. Thangavelu, G. Kannan, S. Backiyarani, T. Arivazhagan, Development of an efficient micropropagation technique for Musa cv. Udhayam (ABB) , Indian Journal of Horticulture: Vol. 71 No. 4 (2014): Indian Journal of Horticulture
- P Murugesan, G Somasundaram, Vikramaditya Pandey, Evaluation of seedlings of oil palm germplasm from Sierra Leone and Senegal in India , Indian Journal of Horticulture: Vol. 78 No. 01 (2021): Indian Journal of Horticulture
- Karan Singh, N. Sharma, Effect of in-situ moisture conservation on morphology, physiology and production of olives under rainfed conditions , Indian Journal of Horticulture: Vol. 67 No. 04 (2010): Indian Journal of Horticulture
- P.K. Jain, Akhilesh Tiwari, Effect of irrigation systems and frequencies on growth and yield of papaya , Indian Journal of Horticulture: Vol. 69 No. 02 (2012): Indian Journal of Horticulture
- Ravi Kher, ahangeer A. Baba, Parshant Bakshi, Influence of planting time and mulching material on growth and fruit yield of strawberry cv. Chandler , Indian Journal of Horticulture: Vol. 67 No. 04 (2010): Indian Journal of Horticulture
- B.M. Kalalbandi, D.P. Waskar, V.S. Khandare, D.S. Gorad, Micropropagation studies on pomegranate var. Bhagwa , Indian Journal of Horticulture: Vol. 71 No. 4 (2014): Indian Journal of Horticulture
- Aparna Veluru, M Neema, S Jayasekhar, Vandita Kumari, V Selvamani, Sapna Panwar, Namita ., Kanwar Pal Singh, P Subramanian, Ravi Bhat, Evaluation of indigenous rose varieties as intercrop under coconut plantation in India , Indian Journal of Horticulture: Vol. 78 No. 03 (2021): Indian Journal of Horticulture
- Pranava Pandey, A.K. Singh, A.K. Dubey, O.P. Awasthi, Effect of salinity stress on growth and nutrient uptake in polyembryonic mango rootstocks , Indian Journal of Horticulture: Vol. 71 No. 01 (2014): Indian Journal of Horticulture
- P.S. Gurjar, G.S. Rana, Influence of foliar application of nutrients and growth regulator on fruit drop, yield and fruit size and quality in Kinnow mandarin , Indian Journal of Horticulture: Vol. 71 No. 01 (2014): Indian Journal of Horticulture
- Abhay K. Gaurav, Effect of coloured shade net on production of Dracaena fragrans , Indian Journal of Horticulture: Vol. 73 No. 1 (2016): Indian Journal of Horticulture
<< < 7 8 9 10 11 12 13 14 15 16 > >>
You may also start an advanced similarity search for this article.
Most read articles by the same author(s)
- S.S. Dey, R. Bhatia Dey, Chander Parkash, Raj Kumar, Heterosis and combining ability analysis in snowball cauliflower using indigenously developed CMS lines , Indian Journal of Horticulture: Vol. 74 No. 03 (2017): Indian Journal of Horticulture
- Reeta Bhatia, S.S. Dey, Raj Kumar, Genetic divergence studies in tulip (Tulipa gesneriana L.) , Indian Journal of Horticulture: Vol. 74 No. 04 (2017): Indian Journal of Horticulture
- S.S Dey, Reeta Bhatia, Chander Parkash, Pritam Kalia, R.N Barwal, Evaluation of cauliflower (Brassica oleracea var. botrytis) CMS (Ogura) lines for agronomic and floral traits , Indian Journal of Horticulture: Vol. 71 No. 03 (2014): Indian Journal of Horticulture
- S.S. Dey, T.K. Behera, A.D. Munshi, S. Rakshit, R. Bhatia, Utility of gynoecious sex form in heterosis breeding of bitter gourd and genetics of associated vegetative and flowering traits , Indian Journal of Horticulture: Vol. 69 No. 04 (2012): Indian Journal of Horticulture
- S.S. Dey, T.K. Behera, A.D. Munshi, Anand Pal, Combining ability analysis in bitter gourd , Indian Journal of Horticulture: Vol. 67 No. 03 (2010): Indian Journal of Horticulture
- Chander Parkash, S.S. Dey, Reeta Bhatia, M.R. Dhiman, Indigenously developed SI and CMS lines in hybrid breeding of cabbage , Indian Journal of Horticulture: Vol. 72 No. 02 (2015): Indian Journal of Horticulture
- Chander Parkash, Inheritance of quantitative characters in knol khol , Indian Journal of Horticulture: Vol. 68 No. 02 (2011): Indian Journal of Horticulture