Effect of different method of placement of shoot tips on in vitro shoot tip grafting in Nagpur mandarin

Abstract

Shoot tip grafting (STG) comprises of in vitro grafting of meristem along with leaf primordia on the rootstock. Success of STG depends upon the method of placement of this meristem. Three different grafting techniques were followed in the present study using Nagpur Mandarin as scion on Rough lemon stock. Sterilized decoated seeds of Rough lemon were germinated in MS medium at 27+ 10C in complete darkness for two weeks. Surface sterilized shoot tips of Nagpur Mandarin of 0.1 to 0.3 mm were placed by top cut surface, window cut and apical triangle cut method, so as to record the success percentage. Although early sprouting of grafts was observed by top cut surface method in 13.25 days with the grafted shoot length of 1.88 cm but maximum success of 46.66% was observed by apical triangle cut method, The successful grafts with 2-4 expanded leaves were transplanted to soilrite for hardening.