Identification of SSR and miRNA from transcriptome of tuberose

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Published

2019-03-13

DOI:

https://doi.org/10.5958/0974-0112.2019.00020.3

Keywords:

Polianthes tuberosa, microsatellites, simple sequence repeats (SSR), microRNA.
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Issue

Vol. 76 No. 01 (2019): Indian Journal of Horticulture

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Articles

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Copyright (c) 2019 Indian J. Hortic.

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This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Authors

  • Jayanthi Madhavan Division of Nematology, ICAR-Indian Agricultural Research Institute, New Delhi 110012
  • Pawan Jayaswal National Research Centre for Plant Biotechnology, Pusa, New Delhi 110012
  • Kanchan BM Singh Division of Nematology, ICAR-Indian Agricultural Research Institute, New Delhi 110012
  • Prasoon Kumar Thakur Institute of Molecular Genetics of the ASCR, Vídeňská 1083, 142 20 Prague 4, Czech Republic
  • Uma Rao Institute of Molecular Genetics of the ASCR, Vídeňská 1083, 142 20 Prague 4, Czech Republic

Abstract

For identification of SSRs, the tuberose transcripts were searched with perl script MISA and a total of 1,656 SSRs in 1,280 transcripts with frequency of one SSR per 9.33 kb of the sequence were identified. In 7876 EST sequence of tuberose, we have identified 87 copies of miRNA. The SSR markers identified in this study can be used for genetic diversity analysis of tuberose and can be used to select tuberose varieties with required traits. The data generated in this study will help in better understanding of the possible role of the transcribed microsatellites and miRNA. Further the data will provide deeper knowledge of genetic mechanisms, evolutionary relationships between tuberose and other plants..

How to Cite

Madhavan, J., Jayaswal, P., Singh, K. B., Thakur, P. K., & Rao, U. (2019). Identification of SSR and miRNA from transcriptome of tuberose. Indian Journal of Horticulture, 76(01), 141–147. https://doi.org/10.5958/0974-0112.2019.00020.3

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