Identification of SSR and miRNA from transcriptome of tuberose
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https://doi.org/10.5958/0974-0112.2019.00020.3Keywords:
Polianthes tuberosa, microsatellites, simple sequence repeats (SSR), microRNA.Issue
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Copyright (c) 2019 Indian J. Hortic.
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Abstract
For identification of SSRs, the tuberose transcripts were searched with perl script MISA and a total of 1,656 SSRs in 1,280 transcripts with frequency of one SSR per 9.33 kb of the sequence were identified. In 7876 EST sequence of tuberose, we have identified 87 copies of miRNA. The SSR markers identified in this study can be used for genetic diversity analysis of tuberose and can be used to select tuberose varieties with required traits. The data generated in this study will help in better understanding of the possible role of the transcribed microsatellites and miRNA. Further the data will provide deeper knowledge of genetic mechanisms, evolutionary relationships between tuberose and other plants..
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