Adventitious shoot regeneration in different explants of six genotypes of tomato

Abstract

A protocol has been standardized for adventitious shoot regeneration from different explants without intervening callus formation in six genotypes of tomato. Maximum shoot bud formation was obtained on a medium supplemented with 2.0mgl-1 BAP. Tomato hybrid TH802 has the highest organogenetic potential as compared to other genotypes. Sub culturing of shoots buds on the same medium led to continuous production of multiple shoots. Regenerated shoots were rooted on hormone free MS basal medium. Plantlets were transferred to the field after hardening in the pots containing sand, soil and farmyard manure (1:1:1) in a green house. The regenerated plants were identical to the in vivo raised plants in agro biological features.