Standardization of micropropagation protocol for grape rootstock Dog Ridge
Downloads
Published
DOI:
https://doi.org/10.5958/0974-0112.2020.00084.5Keywords:
Vitis champini, shoot, proliferation, rooting, acclimatizationIssue
Section
License
Copyright (c) 2020 Indian Journal of Horticulture
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Abstract
In the present study, attempt was made to standardize in vitro micropropagation protocol of grape rootstock Dog Ridge. Among two explants compared (axillary bud and shoot tip), axillary buds registered superior shoot proliferation response (75.81%) compared to shoot tips (40.38%) during January to March in Coimbatore conditions. Full strength Murashige and Skoog (MS) medium supplemented with 6-Benzylaminopurine (BAP) 2.0 mg l-1 produced the highest bud sprouting response of explants. Addition of 0.2 mg l-1 1-Naphthaleneacetic acid (NAA) along with BAP 2.0 mg l-1 was found effective for producing maximum number of lengthier multiple shoots within short time and MS medium fortified with BAP 2.0 mg l-1 and gibberellic acid (GA3) 0.5 mg l-1 resulted in elongated shoots. Half-strength MS medium with 2.0 mg l-1 Indole-3 butyric acid (IBA) and 200 mg l-1 activated charcoal resulted in the maximum in vitro root induction of 83.00% within 11.74 days. For hardening of in vitro rooted plantlets, a combination of pot mix (1:1:1 of sand +soil +farm yard manure) and vermicompost in 1:1 proportion was found to be optimum (86.67% ex vitro survival).
How to Cite
Downloads
Similar Articles
- Taramla Raman, Vidya S Gupta, Susmita Shukla, A robust micropropagation protocol for genetically true to type plants of Phule Arakta pomegranate , Indian Journal of Horticulture: Vol. 76 No. 01 (2019): Indian Journal of Horticulture
- P.R. Meghwal, H.C. Sharma, S.K. Singh, Micropropagation studies on guava , Indian Journal of Horticulture: Vol. 67 No. Special Issue (2010): Indian Journal of Horticulture
- F.A Canlı, F Demir, In vitro multiplication and rooting of ‘F12-1’ (Prunus avium L.) and ‘Maxma 14’ (Prunus mahaleb L. × P. avium L.) rootstocks , Indian Journal of Horticulture: Vol. 71 No. 02 (2014): Indian Journal of Horticulture
- R.G. Somkuwar, P.B. Taware, M.A. Bhange, Rooting behavior and biochemical changes in relation to IBA concentrations in different grape rootstocks , Indian Journal of Horticulture: Vol. 72 No. 02 (2015): Indian Journal of Horticulture
- Rucku Gupta, V K Wali, Parshant Bakshi, Mahital Jamwal, Pramod Kumar, Rafiq Ahmed, Assessment of callus induction and plant regeneration potential of leaf explants derived from in vitro and in vivo shoot of strawberry , Indian Journal of Horticulture: Vol. 77 No. 01 (2020): Indian Journal of Horticulture
- Asha Yadav, Anil K. Poonia, Subhash Kajla, Rapid regeneration and molecular assessment of genetic stability using RAPD markers in stevia , Indian Journal of Horticulture: Vol. 73 No. 02 (2016): Indian Journal of Horticulture
- Jayoti Majumder, Kanwar P. Singh, S.K. Singh, K.V. Prasad, Manjusha Verma, In vitro morphogenesis in marigold using shoot tip as explant , Indian Journal of Horticulture: Vol. 71 No. 01 (2014): Indian Journal of Horticulture
- Deepa Samant, Kundan Kishore, Standardization of pruning for high density Sardar guava orchards under hot and humid climate of Eastern India , Indian Journal of Horticulture: Vol. 76 No. 01 (2019): Indian Journal of Horticulture
- K.L. Kumawat, D.K. Sarolia, A.K. Shukla, Growth, yield and quality of rejuvenated guava as influenced by thinning-bending and micronutrients , Indian Journal of Horticulture: Vol. 69 No. 04 (2012): Indian Journal of Horticulture
- Sujata Upadhyay, M.M. Syamal, Hamidullah Itoo, Micropropagation of sweet orange cv. Mosambi through shoot tips and nodal segments , Indian Journal of Horticulture: Vol. 67 No. Special Issue (2010): Indian Journal of Horticulture
<< < 1 2 3 4 5 6 7 8 9 10 > >>
You may also start an advanced similarity search for this article.
Most read articles by the same author(s)
- Sanchita Ghosh, M. Ganga, K. Soorianathasundaram, Ajit Kumar, Manish Kapoor, Induction of mutation in Jasminum grandiflorum with gamma rays and EMS and identification of novel mutants using molecular markers and SEM imaging , Indian Journal of Horticulture: Vol. 77 No. 04 (2020): Indian Journal of Horticulture
- P. Naveen Kumar, Y.N. Reddy, R. Chandrashekar, Effect of different chemicals on breaking dormancy, growth and flowering of gladiolus cultivars , Indian Journal of Horticulture: Vol. 68 No. 04 (2011): Indian Journal of Horticulture
- Sanchita Ghosh, M. Ganga, K. Soorianathasundaram, Ajit Kumar, Molecular characterization of jasmine genotypes using RAPD and ISSR markers , Indian Journal of Horticulture: Vol. 77 No. 01 (2020): Indian Journal of Horticulture
- P. Murugesan, D. Ramajayam, P. Naveen Kumar, Anitha Pedapati, G. Ravichandran, H. P. Bhagya, Vikramaditya Pandey, Evaluation of wild oil palm germplasm for horticultural traits , Indian Journal of Horticulture: Vol. 77 No. 03 (2020): Indian Journal of Horticulture
- Soobedar Yadav, P. Naveen Kumar, Ajay Arora, Ramesh Kumar, Effect of protease inhibitors on physiological and biochemical changes influencing keeping quality in gladiolus , Indian Journal of Horticulture: Vol. 72 No. 01 (2015): Indian Journal of Horticulture