Standardization of micropropagation protocol for grape rootstock Dog Ridge
Downloads
Published
DOI:
https://doi.org/10.5958/0974-0112.2020.00084.5Keywords:
Vitis champini, shoot, proliferation, rooting, acclimatizationIssue
Section
License
Copyright (c) 2020 Indian Journal of Horticulture
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Abstract
In the present study, attempt was made to standardize in vitro micropropagation protocol of grape rootstock Dog Ridge. Among two explants compared (axillary bud and shoot tip), axillary buds registered superior shoot proliferation response (75.81%) compared to shoot tips (40.38%) during January to March in Coimbatore conditions. Full strength Murashige and Skoog (MS) medium supplemented with 6-Benzylaminopurine (BAP) 2.0 mg l-1 produced the highest bud sprouting response of explants. Addition of 0.2 mg l-1 1-Naphthaleneacetic acid (NAA) along with BAP 2.0 mg l-1 was found effective for producing maximum number of lengthier multiple shoots within short time and MS medium fortified with BAP 2.0 mg l-1 and gibberellic acid (GA3) 0.5 mg l-1 resulted in elongated shoots. Half-strength MS medium with 2.0 mg l-1 Indole-3 butyric acid (IBA) and 200 mg l-1 activated charcoal resulted in the maximum in vitro root induction of 83.00% within 11.74 days. For hardening of in vitro rooted plantlets, a combination of pot mix (1:1:1 of sand +soil +farm yard manure) and vermicompost in 1:1 proportion was found to be optimum (86.67% ex vitro survival).
How to Cite
Downloads
Similar Articles
- Hira Singh, T.S Dhillon, In vitro morphogenesis and plant regeneration response from hot pepper (Capsicum annuum L.) cultivar Punjab Guchhedar , Indian Journal of Horticulture: Vol. 71 No. 03 (2014): Indian Journal of Horticulture
- Rajni Rajan, Muhammad Feza Ahmad, Awadhesh K. Pal, Kuldeep Pandey, Assessment of radiation sensitivity and regeneration in shoot tip culture of banana cv. Grand Naine , Indian Journal of Horticulture: Vol. 80 No. 04 (2023): Indian Journal of Horticulture
- S. Satpathy, Akhilesh Kumar, T.M. Shivalingaswamy, A.B. Rai, Effect of foliar spray of boron on biology, egg laying activity and control of brinjal shoot and fruit borer (Leucinodes orbonalis Guen.) , Indian Journal of Horticulture: Vol. 69 No. 02 (2012): Indian Journal of Horticulture
- Mohinder Kaur, Ajmer S. Dhatt, J.S. Sandhu, S.S. SanGosaldhu, Effect of genotype, explant and culture media on direct plant regeneration in eggplant , Indian Journal of Horticulture: Vol. 72 No. 02 (2015): Indian Journal of Horticulture
- Aroosa Khalil, M.K. Sharma, Nowsheen Nazir, A.S. Sundouri, Effect of bud load and fertilizer application on growth, yield and quality of Sahebi grape , Indian Journal of Horticulture: Vol. 75 No. 03 (2018): Indian Journal of Horticulture
- Zeliha Gökbayrak, Alper Dardeniz, Abdurrahman Arikan, Ulaş Kaplan, Submersion of grape rootstock 41B cuttings in water increase root formation , Indian Journal of Horticulture: Vol. 68 No. 02 (2011): Indian Journal of Horticulture
- Madhurjit Singh Rathore, Pooja Sharma, Vivek Kumar Dhiman, Manisha Thakur, An efficient in vitro propagation of clonal cherry rootstock Gisela-6 and validation of genetic stability through SCoT markers , Indian Journal of Horticulture: Vol. 81 No. 02 (2024): Indian Journal of Horticulture
- Madhurjit Singh Rathore, Pooja Sharma, Vivek Kumar Dhiman, Manisha Thakur, An efficient in vitro propagation of clonal cherry rootstock Gisela-6 and validation of genetic stability through SCoT markers , Indian Journal of Horticulture: Vol. 81 No. 02 (2024): Indian Journal of Horticulture
- S.D. Shikhamany, Sanjay K. Jeughale, Kailas N. Khapre, Response of 2A Clone grape to GA3 sprays for berry thinning as influenced by cane regulation on two training systems under tropical conditions , Indian Journal of Horticulture: Vol. 74 No. 01 (2017): Indian Journal of Horticulture
- Poonam Chaudhary, Pritam Kalia, Raghavendra Aminedi, Shilpi Bansal, Sanjeev Kumar, Optimization of in vitro conditions favourable for effective regeneration in Pusa Meghna Indian cauliflower , Indian Journal of Horticulture: Vol. 77 No. 01 (2020): Indian Journal of Horticulture
<< < 5 6 7 8 9 10 11 12 13 14 > >>
You may also start an advanced similarity search for this article.
Most read articles by the same author(s)
- Sanchita Ghosh, M. Ganga, K. Soorianathasundaram, Ajit Kumar, Manish Kapoor, Induction of mutation in Jasminum grandiflorum with gamma rays and EMS and identification of novel mutants using molecular markers and SEM imaging , Indian Journal of Horticulture: Vol. 77 No. 04 (2020): Indian Journal of Horticulture
- P. Naveen Kumar, Y.N. Reddy, R. Chandrashekar, Effect of different chemicals on breaking dormancy, growth and flowering of gladiolus cultivars , Indian Journal of Horticulture: Vol. 68 No. 04 (2011): Indian Journal of Horticulture
- Sanchita Ghosh, M. Ganga, K. Soorianathasundaram, Ajit Kumar, Molecular characterization of jasmine genotypes using RAPD and ISSR markers , Indian Journal of Horticulture: Vol. 77 No. 01 (2020): Indian Journal of Horticulture
- P. Murugesan, D. Ramajayam, P. Naveen Kumar, Anitha Pedapati, G. Ravichandran, H. P. Bhagya, Vikramaditya Pandey, Evaluation of wild oil palm germplasm for horticultural traits , Indian Journal of Horticulture: Vol. 77 No. 03 (2020): Indian Journal of Horticulture
- Soobedar Yadav, P. Naveen Kumar, Ajay Arora, Ramesh Kumar, Effect of protease inhibitors on physiological and biochemical changes influencing keeping quality in gladiolus , Indian Journal of Horticulture: Vol. 72 No. 01 (2015): Indian Journal of Horticulture