Optimization of in vitro conditions favourable for effective regeneration in Pusa Meghna Indian cauliflower

Abstract

Establishment of an efficient in vitro regeneration system is a pre-requisite for genetic modification of plants. The present study reports a high efficiency regeneration protocol for cauliflower cv. Pusa Meghna using cotyledon and hypocotyl explants cultured on different growth media with varying combinations of plant growth regulators. Maximum shoot regeneration response from hypocotyl (95.76%) and cotyledon (83.01%) was obtained on MS medium containing 1mg/L BAP and 1 mg/L BAP+0.1 mg/L NAA+0.5 mg/L AgNO3+0.1 mg/L GA3, respectively. Further optimization of rooting media for development of full grown plants showed maximum root regeneration of 92.20% and 94.86 % from hypocotyl and cotyledon explants grown on MS medium containing 0.5 mg/L IBA and 0.2 mg/L IBA, respectively. Utilization of the present established regeneration protocol would serve as a valuable tool for various crop improvement studies in cauliflower