QTL mapping for yield traits in vegetable cowpea

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Published

2024-12-30

DOI:

https://doi.org/10.58993/ijh/2024.81.4.8

Keywords:

Linkage map, Marker assisted selection, Microsatellite markers, SSR, Vigna unguiculata
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Issue

Vol. 81 No. 04 (2024): Indian Journal of Horticulture

Section

Research Articles

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Copyright (c) 2024 Indian Journal of Horticulture

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This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

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Authors

  • Ashwin Vargheese Kerala Agricultural University
  • Deepu Mathew Kerala Agricultural University https://orcid.org/0000-0002-2941-1060
  • Jiji Joseph Kerala Agricultural University
  • Pradeepkumar T. Kerala Agricultural University

Abstract

The F4 population comprising of 92 plants descended from the cross of semi-trailing, medium-long podded, low yielding cv. Kanakamony [Vigna unguiculata (L.) ssp. cylindrica] with pole type, long podded, high yielding cv. Sharika [V. unguiculata. ssp. sesquipedalis] was used for mapping the yield-related traits in vegetable type cowpea. Using 30 polymorphic SSR markers, a linkage map spanning 908 cM with two linkage groups (LG1 with eight markers spanning 637 cM and LG2 with five markers spanning 271 cM) was constructed. CLM0083 was an anchored marker for pod weight and total dry pod yield. For days taken for the first flowering, LG1 and LG2 had four and three hotspots, respectively. SMA confirmed that CLM0177 on LG1 and CLM0300 are anchored markers for this trait. For branch number, a hotspot was found on LG2 between CLM0200 and CLM0088. SMA confirmed the linkage of CLM0200 with this trait. For root length, a hotspot was found between CLM0244 and CLM0008 on LG1. On LG2, the hotspots were present between CLM0200 and CLM0088, and between CLM0260 and CLM0218. SMA confirmed the linkage of CLM0201, CLM0244 and CLM0300, with this trait. For plant height, a hotspot existed between markers CLM008 and CLM177 on LG1. For plant weight, a hotspot with anchored marker CLM0244 and flanking markers CLM0186 and CLM0008 was found on LG1. The region 24.25-126.86 cM on LG1, bracketed by CLM0244 and CLM0177 and with an anchored marker CLM0008, had QTL hotspots for days taken for first flowering, total dry pod yield, root length, plant height and plant weight.

How to Cite

Vargheese, A., Mathew, D., Joseph, J., & T., P. (2024). QTL mapping for yield traits in vegetable cowpea. Indian Journal of Horticulture, 81(04), 385–391. https://doi.org/10.58993/ijh/2024.81.4.8

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References

Almeida, W.S., Fernandes, F.R.B., Teófilo, E.M. and Bertini, C.H.C.M. 2014. Correlation and path analysis in components of grain yield of cowpea genotypes. Rev. Ciênc. Agron. 45: 726-36. https://doi.org/10.1590/S1806-66902014000400010

Andargie, M., Pasquet, R.S., Gowda, B.S., Muluvi, G.M. and Timko, M.P. 2011. Construction of a SSR-based genetic map and identification of QTL for domestication traits using recombinant in-bred lines from a cross between wild and cultivated cowpea (V. unguiculata (L.) Walp). Mol. Breed. 28: 413-20. https://doi.org/10.1007/s11032-011-9598-2

Andargie, M., Pasquet, R.S., Muluvi, G.M. and Timko, M.P. 2013. Quantitative trait loci analysis of flowering time related traits identified in recombinant inbred lines of cowpea (Vigna unguiculata). Genome 56: 289-94. https://doi.org/10.1139/gen-2013-0028

Bermejo, C., Cazzola, F., Maglia, F. and Cointry, E. 2020. Selection of parents and estimation of genetic parameters using BLUP and molecular methods for lentil (Lens culinaris Medik.) breeding program in Argentina. Exp. Agric. 56: 12-25. https://doi.org/10.1017/S0014479719000061

Box, G.E.P. and Cox, D.R. 1964. An analysis of transformations. J. R. Stat. Soc. B (Statistical Methodology) 26: 211-43. https://doi.org/10.1111/j.2517-6161.1964.tb00553.x

Doyle, J.J. and Doyle, J.L. 1990. Isolation of plant DNA from fresh tissue. Focus 12: 13-15.

González, A.M., Yuste-Lisbona, F.J., Saburido, S., Bretones, S., De Ron, A.M., Lozano, R. and Santalla, M. 2016. Major contribution of flowering time and vegetative growth to plant production in common bean as deduced from a comparative genetic mapping. Front. Plant Sci. 7: 1940. https://doi.org/10.3389/fpls.2016.01940

Kongjaimun, A., Kaga, A., Tomooka, N., Somta, P., Shimizu, T., Shu, Y., Isemura, T., Vaughan, D.A. and Srinives, P. 2012. An SSR-based linkage map of yard-long bean (Vigna unguiculata (L.) Walp. subsp. unguiculata Sesquipealis Group) and QTL analysis of pod length. Genome 55: 81-92. https://doi.org/10.1139/g11-078

Kongjaimun, A., Somta, P., Tomooka, N., Kaga, A., Vaughan, D.A. and Srinives, P. 2013. QTL mapping of pod tenderness and total soluble solid in yardlong bean [Vigna unguiculata (L.) Walp. subsp. unguiculata cv. sesquipedalis]. Euphytica 189: 217-23. https://doi.org/10.1007/s10681-012-0781-2

Kumar, D.K., Mathew, D., Nazeem, P.A., Abida, P.S. and Thomas, G.C. 2017. A comparative proteome assay on the quality of yardlong bean pods as influenced by the organic and inorganic nourishment systems. Acta Physiol. Plant. 39: 265. https://doi.org/10.1007/s11738-017-2564-9

Mathew, D., Parimelazhagan, T., Gomez, S. and Ahmed, Z. 2007. Characterization of seabuckthorn (Hippophae spp.) genetic resources in India using morphological descriptors. Plant Genet. Resour. Newsl. 149: 22-26.

Pradeepkumar, T., Mathew, D., Varun Roch C., Veni K. and Midhila K.R. 2016. Genetic interrelationship among cowpea varieties elucidated through morphometric, RAPD and SSR analyses. Legume Res. 40: 409-15. https://doi.org/10.18805/lr.v0iOF.3767

Pradhan, D., Mathew, D., Mathew, S.K. and Nazeem, P.A. 2018. Identifying the markers and tagging a leucine-rich repeat receptor-like kinase gene for resistance to anthracnose disease in vegetable cowpea [Vigna unguiculata (L.) Walp.]. J. Hortic. Sci. Biotechnol. 93: 225-31. https://doi.org/10.1080/14620316.2017.1362962

Santos, A.D., Ceccon, G., Davide, L.M.C., Correa, A.M. and Alves, V.B. 2014. Correlations and path analysis of yield components in cowpea. Crop Breed. Appl. Biotechnol. 14: 82-87. https://doi.org/10.1590/1984-70332014v14n2a15

Tanksley, S.D. 1993. Mapping polygenes. Annu. Rev. Genet. 27: 205-33. https://doi.org/10.1146/annurev.ge.27.120193.001225

Watcharatpong, P., Kaga, A., Chen, X. and Somta, P. 2020. Narrowing down a major QTL region conferring pod fiber contents in yardlong bean (Vigna unguiculata), a vegetable cowpea. Genes 11: 363. https://doi.org/10.3390/genes11040363

Xu, P., Wu, X., Wang, B., Hu, T., Lu, Z., Liu, Y., Qin, D., Wang, S. and Li, G. 2013. QTL mapping and epistatic interaction analysis in asparagus bean for several characterized and novel horticulturally important traits. BMC Genet. 14: 4. https://doi.org/10.1186/1471-2156-14-4

Xu, P., Wu, X., Wang, B., Liu, Y., Qin, D., Ehlers, J. D., Close, T. J., Hu, T., Lu, Z. and Li, G. 2010. Development and polymorphism of Vigna unguiculata ssp. unguiculata microsatellite markers used for phylogenetic analysis in asparagus bean (Vigna unguiculata ssp. sesquipedialis (L.) Verdc.). Mol. Breed. 25: 675-84. https://doi.org/10.1007/s11032-009-9364-x

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