Assessment of genetic diversity of grape mutants based on RAPD and SSR markers
Downloads
Published
DOI:
https://doi.org/10.5958/0974-0112.2021.00003.7Keywords:
Genetic diversity, gamma rays, grape mutants, RAPD and SSR markers.Issue
Section
License
Copyright (c) 2021 Indian J. Hortic.

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Abstract
The present investigation was undertaken to assess the genetic diversity in gamma rays irradiated mutants of four grape genotypes based on RAPD and SSR markers. There were 36 mutants selected from in vitro mutated four grape genotypes, namely Pusa Navrang, H-76-1, Pearl of Csaba and Julesky Muscat on the basis of morpho-physiological and biochemical traits. These mutants and non-irradiated individuals were subjected to RAPD and SSR marker analysis to detect the genetic variation amongst them. Six RAPD and eleven SSR primers were employed for PCR amplification and total 63 distinct and easily scoreable bands were generated which ranged between 110 (SSR) and 2500 (RAPD) bp. All the bands were found to be polymorphic in nature and identified 13 solid mutants, among the initially selected 36 putative mutants. The distribution of mutants by cluster analysis suggested existence of high degree of difference at the genetic level in the mutants and non-irradiated control individuals. The mutants were largely grouped into the four major clusters based on the doses of gamma irradiation except some mutants from Julesky Muscat and Pearl of Csaba genotypes. Minimum similarity coefficient (0.37) was noted in mutants of Pusa Navrang and maximum (0.70) in Hybrid-76-1. Unique banding patterns were amplified by both the types of markers (RAPD and SSR). It was suggested that Julesky Muscat was amplified uniquely by RAPD-OPA01, OPP02, SSR-VVMD14, VMCIB11, VMC8G9 primers, H-76-1 by RAPD- OPA01 and SSR- VVMD 14 primers, Pusa Navrang by RAPD-OPP02 and SSR-VVMD-21 primers and Pearl of Csaba by RAPD-OPA01, OPP04, SSR-VMC8G9 primers. The result from this study suggests that OPA01 (RAPD) and VVMD14 (SSR) were the most informative primers and generated maximum numbers of reproducible bands. The combination of these primers will be very useful for the identification of solid mutants which could be evaluated in the field.
How to Cite
Downloads
Similar Articles
- Dimpy Raina, W.S. Dhillon, P.P.S. Gill, Molecular marker-based characterization and genetic diversity of pomegranate genotypes , Indian Journal of Horticulture: Vol. 70 No. 04 (2013): Indian Journal of Horticulture
- Jitendra Kumar, Gyan P. Mishra, A.A. Murkute, G. Phanikumar, Pradeep K. Naik, R.B. Srivastava, Exploring genetic relationships in Artemisia species growing in trans-Himalayan cold arid desert using RAPD markers , Indian Journal of Horticulture: Vol. 69 No. 02 (2012): Indian Journal of Horticulture
- Gunjeet Kumar, N. Sivaraj, V. Kamala, K.K. Gangopadhyay, Sushil Pandey, Shailesh K. Tiwari, N.S. Panwar, B.L. Meena, M. Dutta, Diversity analysis in eggplant germplasm in india using DIVA-GIS approach , Indian Journal of Horticulture: Vol. 70 No. 04 (2013): Indian Journal of Horticulture
- Sanchita Ghosh, M. Ganga, K. Soorianathasundaram, Ajit Kumar, Manish Kapoor, Induction of mutation in Jasminum grandiflorum with gamma rays and EMS and identification of novel mutants using molecular markers and SEM imaging , Indian Journal of Horticulture: Vol. 77 No. 04 (2020): Indian Journal of Horticulture
- S. Lal, A.K. Singh, S.K. Singh, M. Srivastav, N.K. Singh, Genetic diversity in mango cultivars revealed by SSR markers , Indian Journal of Horticulture: Vol. 76 No. 03 (2019): Indian Journal of Horticulture
- Shilpy Kumari, Akash Sharma, Parshant Bakshi, Romesh Salgotra, Manish Sharma, Vishal Gupta, G.K. Rai, Characterization of acid lime genotypes using SSR markers , Indian Journal of Horticulture: Vol. 79 No. 4 (2022): Indian Journal of Horticulture
- Dimpy Raina, W.S. Dhillon, Kuldeep Singh, Analysis of genetic diversity in pear germplasm using morphological traits and DNA markers , Indian Journal of Horticulture: Vol. 68 No. 03 (2011): Indian Journal of Horticulture
- Hemlata Bharti, K.P. Singh, Rakesh Singh, Rajesh Kumar, M.C. Singh, Genetic diversity and relationship study of single and double petal tuberose (Polianthes tuberosa L.) cultivars based on RAPD and ISSR markers , Indian Journal of Horticulture: Vol. 73 No. 02 (2016): Indian Journal of Horticulture
- Xiaohua Du, Deyuan Wang, Zhenhui Gong, Comparison of RSAP, SRAP and SSR markers for genetic analysis in hot pepper , Indian Journal of Horticulture: Vol. 67 No. 04 (2010): Indian Journal of Horticulture
- Khushboo Azam, Hidayatullah Mir, Bishun Deo Prasad, Feza Ahmed, Abha Kumari, Microsatellite marker based characterization of mango cultivars , Indian Journal of Horticulture: Vol. 76 No. 04 (2019): Indian Journal of Horticulture
<< < 1 2 3 4 5 6 7 8 9 10 > >>
You may also start an advanced similarity search for this article.
Most read articles by the same author(s)
- V Palanichamy, Room Singh, S.K Singh, Manish Srivastav, Changes in the reducing and non-reducing sugars during fruit bud differentiation in mango hybrids , Indian Journal of Horticulture: Vol. 71 No. 02 (2014): Indian Journal of Horticulture
- Rahul Dev, S.K. Singh, A.K. Singh, M.K. Verma, Kalpana M, In vitro radio-sensitivity (Gamma irradiation) of some juice and wine making grape genotypes , Indian Journal of Horticulture: Vol. 73 No. 03 (2016): Indian Journal of Horticulture
- Kamlesh Kumar, Manish Srivastav, S.K. Singh, Ankit Singh, Nimisha Sharma, Studies on extent of polyembryony in salt tolerant mango rootstocks , Indian Journal of Horticulture: Vol. 75 No. 01 (2018): Indian Journal of Horticulture
- Kalpana Motha, S.K. Singh, Rakesh Singh, Chet Ram, Manish Srivastav, M.K. Verma, M. Alizadeh, Ch. Bhardwaj, Rahul Dev, Comparative in vitro propagation of stress tolerant grape (Vitis spp.) rootstocks and assessment of clonal fidelity of plantlets , Indian Journal of Horticulture: Vol. 74 No. 03 (2017): Indian Journal of Horticulture
- Rahul Dev, Shailesh Tripathi, V.K. Sah, Rajni Tiwari, Effect of plant density on vegetative growth, tuber yield and essential oil content in Coleus barbatus , Indian Journal of Horticulture: Vol. 70 No. 01 (2013): Indian Journal of Horticulture
- Rajni Tiwari, Lalit Bhatt, Rahul Dev, Effect of date of sowing on growth and yield of vegetable pea genotypes under rain-fed mid-hill conditions of Uttarakhand , Indian Journal of Horticulture: Vol. 71 No. 02 (2014): Indian Journal of Horticulture
- V.B Mhetre, V.B Patel, S.K Singh, M.K Verma, Chavlesh Kumar, G.P Mishra, Anil Dahuja, Atul Kumar, Influence of new generation plant bio-regulators on physio-biochemical alterations in grapes cv. Beauty Seedless , Indian Journal of Horticulture: Vol. 78 No. 02 (2021): Indian Journal of Horticulture
- Anshuman Singh, Jai Prakash, Manish Srivastav, S.K Singh, O.P Awasthi, A.K Singh, S.K Chaudhari, D.K Sharma, Physiological and biochemical responses of citrus rootstocks under salinity stress , Indian Journal of Horticulture: Vol. 71 No. 02 (2014): Indian Journal of Horticulture
- Prativa Lakhotia, Krishan Pal Singh, S.K Singh, M.C Singh, K.V Prasad, Kishan Swaroop, Influence of biotic and abiotic elicitors on production of betalain pigments in bougainvillea callus cultures , Indian Journal of Horticulture: Vol. 71 No. 03 (2014): Indian Journal of Horticulture
- Rahul Dev, Gulshan K. Sharma, Traloki Singh, Effect of gibberellic acid and oxalic acid on colour retention and storage quality of cold stored fruits of ber cv. Gola , Indian Journal of Horticulture: Vol. 75 No. 01 (2018): Indian Journal of Horticulture