Comparative in vitro propagation of stress tolerant grape (Vitis spp.) rootstocks and assessment of clonal fidelity of plantlets
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https://doi.org/10.5958/0974-0112.2017.00065.2Keywords:
Clonal fidelity, comparative multiplication, grape rootstocks, in vitro propagation.Issue
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Copyright (c) 2017 Indian J. Hortic.
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Abstract
Micropropagation in nine Vitis rootstocks using nodal segments was studied. Optimum in vitro culture establishment was highest on Murashige and Skoog (1962) medium (MS) with 3.0 mgl-1 BAP + 0.25 mgl-1 NAA. Rootstock 110 R gave the earliest bud sprouting (4.03 days), whereas, 1616 C and 110 R gave the highest culture establishment (71.11 & 69.67%). The shoot proliferation was most efficient on MS medium + 4.0 mgl-1 IBA + 0.5 mgl-1 BAP. Dogridge showed the maximum multiplication rate/ sub-culture (10.07), while 1613 had the minimum (5.07). Half-strength MS medium supplemented with 4.0 mgl-1 IBA gave good rooting parameters, while halfstrength MS medium with 1.5 mgl-1 IBA + 1.5 mgl-1 NAA induced more number of roots. Dogridge and Salt Creek had the higher rooting (77.58 & 74.24%) compared to other genotypes. High ex vitro plantlet survival (82.75%) was noted in 1103 P in glass jars, while 1616 C plantlets took the shortest time (44.40 days) for transfer to glasshouse. Application of two marker (RAPD & ISSR) systems further confirmed the genetic stability of micropropagated plantlets. Based on the overall performance of rootstocks for in vitro multiplication they could be ranged as Dogridge > Salt Creek > V. parviflora > St. George >1616C > 1103P > 140Ru > 110R > 1613C.
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