Comparative in vitro propagation of stress tolerant grape (Vitis spp.) rootstocks and assessment of clonal fidelity of plantlets

Published

2022-10-13

DOI:

https://doi.org/10.5958/0974-0112.2017.00065.2

Keywords:

Clonal fidelity, comparative multiplication, grape rootstocks, in vitro propagation.
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Authors

  • Kalpana Motha Division of Fruits and Horticultural Technology, ICAR-Indian Agricultural Research Institute, New Delhi 110 012
  • S.K. Singh Division of Fruits and Horticultural Technology, ICAR-Indian Agricultural Research Institute, New Delhi 110 012
  • Rakesh Singh Division of Fruits and Horticultural Technology, ICAR-Indian Agricultural Research Institute, New Delhi 110 012
  • Chet Ram Division of Fruits and Horticultural Technology, ICAR-Indian Agricultural Research Institute, New Delhi 110 012
  • Manish Srivastav Division of Fruits and Horticultural Technology, ICAR-Indian Agricultural Research Institute, New Delhi 110 012
  • M.K. Verma Division of Fruits and Horticultural Technology, ICAR-Indian Agricultural Research Institute, New Delhi 110 012
  • M. Alizadeh Division of Fruits and Horticultural Technology, ICAR-Indian Agricultural Research Institute, New Delhi 110 012
  • Ch. Bhardwaj Division of Fruits and Horticultural Technology, ICAR-Indian Agricultural Research Institute, New Delhi 110 012
  • Rahul Dev Division of Fruits and Horticultural Technology, ICAR-Indian Agricultural Research Institute, New Delhi 110 012

Abstract

Micropropagation in nine Vitis rootstocks using nodal segments was studied. Optimum in vitro culture establishment was highest on Murashige and Skoog (1962) medium (MS) with 3.0 mgl-1 BAP + 0.25 mgl-1 NAA. Rootstock 110 R gave the earliest bud sprouting (4.03 days), whereas, 1616 C and 110 R gave the highest culture establishment (71.11 & 69.67%). The shoot proliferation was most efficient on MS medium + 4.0 mgl-1 IBA + 0.5 mgl-1 BAP. Dogridge showed the maximum multiplication rate/ sub-culture (10.07), while 1613 had the minimum (5.07). Half-strength MS medium supplemented with 4.0 mgl-1 IBA gave good rooting parameters, while halfstrength MS medium with 1.5 mgl-1 IBA + 1.5 mgl-1 NAA induced more number of roots. Dogridge and Salt Creek had the higher rooting (77.58 & 74.24%) compared to other genotypes. High ex vitro plantlet survival (82.75%) was noted in 1103 P in glass jars, while 1616 C plantlets took the shortest time (44.40 days) for transfer to glasshouse. Application of two marker (RAPD & ISSR) systems further confirmed the genetic stability of micropropagated plantlets. Based on the overall performance of rootstocks for in vitro multiplication they could be ranged as Dogridge > Salt Creek > V. parviflora > St. George >1616C > 1103P > 140Ru > 110R > 1613C.

How to Cite

Motha, K., Singh, S., Singh, R., Ram, C., Srivastav, M., Verma, M., Alizadeh, M., Bhardwaj, C., & Dev, R. (2022). Comparative in vitro propagation of stress tolerant grape (Vitis spp.) rootstocks and assessment of clonal fidelity of plantlets. Indian Journal of Horticulture, 74(03), 317–325. https://doi.org/10.5958/0974-0112.2017.00065.2

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