Physiological basis of post-harvest ripening and standardization of seed extraction in ash gourd

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Published

2023-06-28

DOI:

https://doi.org/10.58993/ijh/2023.80.2.4
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Vol. 80 No. 2 (2023): Indian Journal of Horticulture

Section

Research Articles

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Copyright (c) 2023 Indian Journal of Horticulture

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This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Authors

  • Nakul Gupta ICAR-Indian Institute of Vegetable Research, Varanasi-221305, Uttar Pradesh, India
  • P.M. Singh ICAR-Indian Institute of Vegetable Research, Varanasi-221305, Uttar Pradesh, India
  • Rajesh Kumar ICAR-Indian Institute of Vegetable Research, Varanasi-221305, Uttar Pradesh, India
  • Tribhuvan Chaubey ICAR-Indian Institute of Vegetable Research, Varanasi-221305, Uttar Pradesh, India
  • Vikas Singh ICAR-Indian Institute of Vegetable Research, Varanasi-221305, Uttar Pradesh, India

Abstract

Manual seed extraction of ash gourd is time and labour extensive, and freshly harvested seeds possess dormancy. Hence, seed extraction protocol was standardized, and physio-biochemical changes associated with post-harvest ripening (PHR) were elucidated in ash gourd cv. Kashi Dhaval. Allowing pulp to ferment for 24 to 48 h gave the best quality seed (germination and vigour) compared to acid, alkali and manual extraction. PHR of fruit for 20-30 days before seed extraction gave the higher seed yield (seeds per fruit and 100-seed weight), higher seed germination and vigour compared to seed obtained from freshly harvested fruits. Physiological analysis showed that PHR increased the seed reserve (total soluble proteins, starch) except total soluble sugars, conceivably due to conversion to starch. Decreased O2 and H2 O2 activity in PHR seeds indicated a lower threat to oxidative stress, probably due to decreased seed moisture. Consequently, SOD, CAT and POD activity was reduced in PHR seeds. In conclusion, allowing fruit pulp to ferment for 24 to 48 h gave the best quality seed during seed extraction. Moreover, 20-30 days PHR in ash gourd is recommended because numerous physiobiochemical changes related to dormancy release and germination enhancement occur during PH

How to Cite

Gupta, N., Singh, P. M., Kumar, R., Chaubey, T., & Singh, V. (2023). Physiological basis of post-harvest ripening and standardization of seed extraction in ash gourd . Indian Journal of Horticulture, 80(2), 153–158. https://doi.org/10.58993/ijh/2023.80.2.4

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References

Bailly, C. 2019.The signalling role of ROS in

regulating seed germination and dormancy.

Biochem. J. 476: 3019-32.

Bimakar, M., Rahman, R.A., Taip, F.S.,

Adzahan, N.M., Sarker Z.I. and Ganjloo, A.

Optimization of ultrasound-assisted

crude oil e from winter melon (Benincasa

hispida) seed using response surface

methodology and evaluation of its antioxidant

activity, total phenolic content and fatty acid

composition. J. Mol.17:11748-62.

Chaitanya, K.S.K. and Naithani, S.C. 1994.

Role of superoxide, lipid peroxidation

and superoxide dismutase in membrane

perturbation during loss of viability in seeds

of Shorea robusta Gaertn F. New Phytol. 126:

-27.

Dhindsa, R.S., Plumb-Dhindsa, P. and Thorpe,

T.A. 1981. Leaf senescence: Correlated with

increased levels of membrane permeability

and lipid peroxidation, and increased levels of

superoxide dismutase and catalase. J. Exp.

Bot. 32: 93-101.

Finch-Savage, W.E. and Leubner-Metzger,

G. 2006. Seed dormancy and the control of

germination. New Phytol. 171: 501-23.

Ganar, H.D., Kant, K., Dadlani, M., Sihohi, P.S.

and Tomar, B.S. 2004. Effect of after-ripening

and seasons on seed quality of ash gourd

(Benincasa hispida (Thunb.) Cogn.). Seed Res.

:145-148

Gupta, N., Kumar, S., Jain, S.K., Tomar, B.S.,

Singh, J. and Sharma, V. 2021. Effects of stage

of harvest and post-harvest ripening of fruits on

seed yield and quality in cucumber grown under

open field and protected environments. Int. J.

Curr. Microbiol. App. Sci. 10: 2119-34.

ISTA. 2019. International Rules for Seed Testing.

International Seed Testing Association-8303

Barsserssorf, CH-Switzerland.

Kittock, D.L. and Law, A.G.1968. Relationship

of seedling vigour to respiration and tetrazolium

reduction in germinating wheat seeds. Agron. J.

: 268-88.

Manimurugan, C., Pandita, V.K., Tomar, B.S.,

Gupta, N. and Singh, P.M. 2017. Effect of plant

density and foliar nutrient application on seed

yield and quality in Asiatic carrot (Dacus carota).

Indian J. Agric. Sci. 87: 419-24.

Mukherjee, S.P. and Choudhari, M.A. 1983.

Implications of water stressed induced changes

in the levels of endogenous ascorbic acid and

hydrogen peroxide in Vigna seedlings. Physiol.

Plant. 58:166-70.

Murugesan, P. and Vanangamudi, K. 2005.

Effect of post-harvest fruit storage on seed

quality in ash gourd (Benincasa hispida (Thvnb)

Cogn). Seed Res. 33:160-64.

Pandita, V.K. and Nagarajan, S. 2006. Role

of sugars and proteins in development of

desiccation tolerance in fresh and shade-dried

onion seeds. Aust. J. Exp. Agric. 46: 1225-30.

Rao, M.V., Paliyathm, G. and Ormond,

D.P. 1996. Ultraviolet-B and ozone induced

biochemical changes in antioxidant enzymes

in Arabidopsis thaliana. Plant Physiol. 110:

-36.

Silva, P.P., Barros, A.C.S.A., Dias, D.C.F.S.,

Sekita, M.C. and Nascimento, W.M. 2017.

Biochemical changes in hybrid pumpkin seeds

at different stages of maturation. Rev. Ciênc.

Agron. 48: 558-64.