An efficient protocol for shoot organogenesis and plant regeneration in jackfruit
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Artocarpus heterophyllus, micropropagation, BAP, GA3, plant growth regulatorsIssue
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Abstract
The present investigation was carried out for the rapid clonal mass multiplication of disease free plants of jackfruit cv. Pant Garima. The nodal explants were collected in winter season proved to be the best with regard to maximum survival of explants. The effect of sterilants (HgCl2 and ethanol) in various combinations was also assessed on survival percentage of explants. The MS medium was supplemented with five concentrations of BAP (1.0, 2.0, 3.0, 4.0 and 5.0 mgL-1) along with control (0.0mgL-1) and their effects on shootlets regeneration, and length of shootlets were evaluated. Maximum shoot proliferation was obtained in the medium supplemented with 4.0 mgL-1 BAP. Further, GA3 @ 1.0 mgL-1 added to MS medium supplemented with 4.0 mgL-1 BAP gave the best results for enhancing the length of the shootlets. For rooting, half strength of MS liquid medium fortified with 2.0 mgL1 IBA gave maximum rooting.
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