Preliminary screening of citrus hybrids for identifying tolerance to Phytophthora nicotianae

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Published

2024-03-30

DOI:

https://doi.org/10.58993/ijh/2024.81.1.5

Keywords:

Citrus, in vitro leaf inoculation, hybrids, necrosis, Phytophthora tolerance
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Vol. 81 No. 01 (2024): Indian Journal of Horticulture

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Articles

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Copyright (c) 2024 Indian Journal of Horticulture

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This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Authors

  • Harsimran Singh Department of Fruit Science, Punjab Agricultural University, Ludhiana 141004, Punjab, India
  • Anita Arora Department of Fruit Science, Punjab Agricultural University, Ludhiana 141004, Punjab, India
  • Krishan Kumar Department of Fruit Science, Punjab Agricultural University, Ludhiana 141004, Punjab, India

Abstract

Phytophthora is one of the most devastating pathogens affecting citrus production. Breeding resistant genotypes is a durable strategy to manage this pathogen. In this study, in vitro screening of 31 citrus hybrids was done against Phytophthora nicotianae using detached leaf assay. The hybrids tested were developed in twoway crosses between Rangpur lime (RL, Phytophthora sensitive) and sour orange (SO, Phytophthora tolerant). The relative Phytophthora sensitivity was determined from the necrotic lesion area after three days of pathogen inoculation. The parents and hybrids could be differentiated into five resistance classes on the basis of necrotic lesion size and area. Among the 18 SO × RL (SR) hybrids, three hybrids viz. SR 3, SR 10 and SR 19 exhibited the least leaf necrotic length (2.8-3.7 mm), breadth (3.2-3.9 mm) and area (9.2-15.0 mm2), indicating a highly resistant reaction. From the 13 RL × SO (RS) hybrids, RS 6 and RS 22 showed highly resistant reaction by virtue of the least necrotic area (12.7-17.9 mm2). Twelve SR and four RS hybrids produced necrotic lesions of >216.8 mm2 area and were rated as highly susceptible in this assay. The parents- Rangpur lime and sour orange recorded highly susceptible and resistant reaction, respectively, which authenticated the results of the leaf assay. The five highly resistant hybrids identified in this study after ex vitro validation of Phytophthora tolerance should prove helpful in commercial citriculture.

How to Cite

Singh, H., Arora, A., & Kumar, K. (2024). Preliminary screening of citrus hybrids for identifying tolerance to Phytophthora nicotianae. Indian Journal of Horticulture, 81(01), 32–36. https://doi.org/10.58993/ijh/2024.81.1.5

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Author Biographies

Harsimran Singh, Department of Fruit Science, Punjab Agricultural University, Ludhiana 141004, Punjab, India

M.Sc. student

Krishan Kumar, Department of Fruit Science, Punjab Agricultural University, Ludhiana 141004, Punjab, India

Senior Plant Pathologist (Fruits)

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