Impact of cold acclimation on the oxidative and anti-oxidative system of bitter gourd seedlings exposed to low-temperature stress

Downloads

Published

2024-12-30

DOI:

https://doi.org/10.58993/ijh/2024.81.4.12

Keywords:

Enzymatic antioxidant, Lipid peroxidation, Non-enzymatic antioxidant, Oxidative parameters, Temperature treatment
Dimensions Badge

Issue

Vol. 81 No. 04 (2024): Indian Journal of Horticulture

Section

Research Articles

License

Copyright (c) 2024 Indian Journal of Horticulture

Creative Commons License

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Crossref
Scopus
Google Scholar
Europe PMC

Authors

  • Veena Devi Ph.D
  • Manjeet Kaur Sangha Ph.D.
  • Sonia Devi Department of Horticulture, Rani Lakshmibai Central Agricultural University, Jhansi, Uttar Pradesh, 284003, India
  • Parminder Kumar Department of Biochemistry, College of Basic Sciences and Humanities, Punjab Agricultural University, Ludhiana, 141004, Punjab, India
  • Ritesh ICAR-Indian Institute of Maize Research, PAU Campus, Ludhiana, Punjab, 141004, India
  • Mamta Pathak Department of Vegetable Sciences, College of Horticulture and Forestry, Punjab Agricultural University, Ludhiana, Punjab, 141004, India

Abstract

Bitter gourd (Momordica charantia L.) is a cold-sensitive flowering vine, but it remains in demand around the year due to its medicinal properties. Therefore, a prime need is there to develop a cold resilient bitter gourd genotype. The study was designed to inspect the impact of cold stress and acclimation on oxidative and anti-oxidative parameters in two bitter gourd genotypes viz., PAUBG-56 and Punjab-14. For that fifteen-dayold bitter gourd seedlings were subjected to low-temperature stress (5°C) both directly and after acclimation (gradual decrease in temperature from 25 to 5°C before cold exposure). Leaflets were collected on various days after temperature treatment, and seven days of recovery period and analyzed for the above-mentioned parameters. Results showed a significant increase in oxidative stress markers in non-acclimated Punjab-14 seedlings, while PAUBG-56 demonstrated higher resilience through elevated enzymatic and non-enzymatic antioxidant activity. Acclimation improved stress tolerance in both genotypes, although PAUBG-56 showed superior recovery. Therefore, it is concluded that acclimation is an eminent method, that helps to enhance the tolerance capacity of bitter gourd against low-temperature stress.

How to Cite

Devi, V., Sangha, M. K., Devi, S., Kumar, P., Ritesh, & Pathak, M. (2024). Impact of cold acclimation on the oxidative and anti-oxidative system of bitter gourd seedlings exposed to low-temperature stress. Indian Journal of Horticulture, 81(04), 417–424. https://doi.org/10.58993/ijh/2024.81.4.12

Downloads

Download data is not yet available.

Author Biography

Manjeet Kaur Sangha, Ph.D.

She is working as a Principal Biochemist-cum-Head, Department of Biochemistry, Punjab Agricultural University.

References

Aebi, H.E. 1983. Catalase. In: Methods of Enzymatic Analysis, Vol. III. H.O. Bergmeyer (Ed.) Academic Press, New York, pp. 273-286.

Airaki, M., Leterrier, M., Mateos, R.M., Valderrama, R., Chaki, M. and Barroso, J. 2012. Metabolism of reactive oxygen species and reactive nitrogen species in pepper (Capsicum annum L.) plants under low temperature stress. Plant Cell Environ. 35: 281-295. https://doi.org/10.1111/j.1365-3040.2011.02310.x

Behera, T.K., Behera, S. and Bharathi, L.K. 2010. Bitter Gourd: Botany, Horticulture, Breeding. Hort. Rev. 37: 101-141. https://doi.org/10.1002/9780470543672.ch2

Beutler, E., Durron, O. and Kelly, B.M. 1963. Improved method for the determination of blood glutathione. J. Lab Clin. Med. 61: 882-888.

Chinard, F.P. 1952. Photometric estimation of proline and ornithine. J. Biol. Chem. 199: 91-95.

Claiborne, S. and Fridovich, I. 1979. Assay for peroxidase. In: Biochemical Methods, S. Sadasivam, Manickam (Ed.), New Age International Publishers, New Delhi, pp. 190.

Devi, V., Sangha, M.K., Pathak, M. and Kumar, P. 2021. Biochemical changes in bitter gourd in response to low temperature stress. Indian J. Hort. 78: 78-83. https://doi.org/10.5958/0974-0112.2021.00011.6

Dhindsa, R.S. and Matowe, W.,1981. Drought tolerance in two mosses: correlated with enzymatic defence against lipid peroxidation. J. Exp. Bot. 32: 79-91. https://doi.org/10.1093/jxb/32.1.79

Fahimirad, S., Karimzadeh, G. and Ghanati, F. 2013. Cold-induced changes of antioxidant enzymes activity and lipid peroxidation in two canola (Brassica napus L.) cultivars. J. Plant Physiol. Breed. 3: 1.

Gong, M., Li, H.Y. and Li, C.G. 2011. Short term cold shock at 1˚C induced chilling tolerance in maize seedlings. IACSIT Press: Singapore, 1: 346. doi:10.1016/j.egypro.200.10.519

Hajiboland, R. and Habibi, G. 2011. Contrastive responses of spring and winter wheat cultivars to chilling and acclimation treatments. Acta. Agri. Sloven. 97: 233-239.

Jagota, S.K. and Dani, H.M. 1982. A new colorimetric technique for estimation of vitamin C using folin phenol reagent. Anal. Biochem. 15: 178-182. doi: 10.1016/0003-2697(82)90162-2

Jiang, A., Guo, Y.J., Fan, Y., Xiang, B.J., He, W., Wang, L. and Zhang, J. 2010. Effect of low temperature stress on cold resistance of Zea mexicana seedling. Pratacultural Sci. 27: 89-92.

Li, S.L., Xia, Y.Z. and Liu, J. 2014. Effects of cold-shock on tomato seedlings under high-temperature stress. Chin. J. Appl. Ecol. 25: 2927-2934.

Liu, W., Yu, K., He, T., Li, F., Zhang, D. and Liu, J. 2013. The low temperature induced physiological responses of Avena nuda L., a cold-tolerant plant species. Sci. World J. 2013: 658793. https://doi.org/10.1155/2013/658793

Mensor, L.L., Menezes, F.S., Leita, G.G., Reis, A.S., Santos, T.C., Coube, C.S. and Leita, S.G. 2001. Screening of brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phyto. Res. 15: 127-130. doi: 10.1002/ptr.687. PMID: 11268111

Mo, Y., Liang, G., Shi, W. and Xie, J. 2010. Metabolic responses of alfalfa (Medicago Sativa L.) leaves to low and high temperature induced stresses. Afr. J. Biotech. 10: 1117-1124.

Singh, B.K., Sutradhar, M., Singh, A.K. and Mandal, N. 2017. Cold stress in rice at early growth stage – An overview. Int. J. Pure App. Biosci. 5: 407-419. https://dx.doi.org/10.18782/2320-7051.2750

Sinha, A.K. 1971. Colorimetric assay of catalase. Anal. Biochem. 47: 389-394. doi: 10.1016/0003-2697(72)90132-7

Xing, Z., Wang, Y., Feng, Z. and Tan, Q. 2008. Effect of different packaging films on postharvest quality and selected enzyme activities of Hypsizygus marmoreus mushrooms. J. Agri. Food Chem. 56: 11838-11844. doi: 10.1021/jf8024387

Yadegari, L.Z., Heidari, R. and Carapetian, J. 2007. The influence of cold acclimation on proline, malondialdehyde (MDA), total protein and pigments contents in soybean (Glycine max) seedlings. J. Biol. Sci. 7: 1436-1441. doi:10.3923/jbs.2007.1436.1441

Zuther, E., Juszczak, L., Lee, Y.P., Baier, M. and Hincha, D.K. (2015). Time-dependent deacclimation after cold acclimation in Arabidopsis thaliana an accessions. Sci. Rep. 5: 1-10. https://doi.org/10.1038/srep12199

Similar Articles

<< < 4 5 6 7 8 9 10 11 12 13 > >> 

You may also start an advanced similarity search for this article.