DNA fingerprinting of aonla varieties using RAPD markers

Abstract

Aonla (Emblica officinalis L.) is known for its medicinal and therapeutic properties from the ancient time in India and considered as wonder fruit for health conscious population. DNA fingerprinting for cultivar or varietal identification has become important tool for genetic identification in plant breeding and germplasm management. With aim to understand relationship among different popular aonla cultivars developed by N.D.U.A.&T., Faizabad and popular ones existing in the region, fingerprinting using RAPD markers was undertaken. DNA was isolated from young leaves of ten aonla cultivars. Six RAPD markers (OPA-7, OPA-8, OPZ -4, OPZ-14, OPY-1and OPY-2) were used for the analysis for genetic variation. Out of six primers, OPZ-14 gave most distinct banding pattern for cv. Banarasi. Dendrogram represented four major groups, which was further divided into sub-groups IA and IB. Sub-group IA had NA-04 and NA-10, while IB had cv. Francis. Cluster IIA represented NA-5, NA-6 and NA-9, while IIB represents NA-7 and Chakaiya. Banarasi and Anand represented by group III and IV respectively.