Comparison of fluorescein- and 32P-labelled probes for safe detection of potato spindle tuber viroid in potato

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Published

2012-04-09

Keywords:

Fluorescein, 32P-labelled, probe, potato spindle tuber viroid.
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Issue

Vol. 69 No. 01 (2012): Indian Journal of Horticulture

Section

Articles

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Copyright (c) 2012 Indian Journal of Horticulture

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This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Authors

  • Priyanka Kaundal Central Potato Research Institute, Shimla 171 001, Himachal Pradesh
  • Jagesh K. Tiwari Central Potato Research Institute, Shimla 171 001, Himachal Pradesh
  • Nimisha Kaushal Central Potato Research Institute, Shimla 171 001, Himachal Pradesh
  • Deep Kumar Central Potato Research Institute, Shimla 171 001, Himachal Pradesh
  • J. Gopal Central Potato Research Institute, Shimla 171 001, Himachal Pradesh
  • S.K. Pandey Central Potato Research Institute, Shimla 171 001, Himachal Pradesh
  • I.D. Garg Central Potato Research Institute, Shimla 171 001, Himachal Pradesh

Abstract

Two methods namely fluorescein- and 32P-labelled probes were tested for detection of Potato Spindle tuber viroid in potato. Out of 94 potato microplants tested, 82 were found positive, while 12 were virus-free. On comparison with NASH, it could be seen that fluorescein-labelled probe was quite cost effective.

How to Cite

Kaundal, P., Tiwari, J. K., Kaushal, N., Kumar, D., Gopal, J., Pandey, S., & Garg, I. (2012). Comparison of fluorescein- and 32P-labelled probes for safe detection of potato spindle tuber viroid in potato. Indian Journal of Horticulture, 69(01), 121–124. Retrieved from https://journal.iahs.org.in/index.php/ijh/article/view/1712

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