Identification of host gene conferring resistance to Potato virus Y using Ry gene-based molecular markers

Published

2013-09-27

Keywords:

MAS, molecular markers, potato, PVY resistance, Ry gene.
Dimensions Badge

Authors

  • Jagesh K. Tiwari Central Potato Research Institute, Shimla, Himachal Pradesh 171 001
  • Poonam . Central Potato Research Institute, Shimla, Himachal Pradesh 171 001
  • S.K. Chakrabarti Central Potato Research Institute, Shimla, Himachal Pradesh 171 001
  • Vinod Kumar Central Potato Research Station, Kufri, Shimla, Himachal Pradesh 171 012
  • J. Gopal DOR on Onion and Garlic, Rajgurunagar, Pune
  • B.P. Singh Central Potato Research Institute, Shimla, Himachal Pradesh 171 001
  • S.K. Pandey Central Potato Research Institute, Shimla, Himachal Pradesh 171 001
  • D. Pattanayak Central Potato Research Institute, Shimla, Himachal Pradesh 171 001

Abstract

Potato (Solanum tuberosum L.) is the third most important food crop of the world and vegetatively propagated mainly through tubers. Tuber yield mainly depends on the quality of potato ‘seed tubers’ especially free from viral diseases, otherwise it proliferates over the successive clonal generations. More than 30 different viruses infect potato, and potato virus Y (PVY) is the most important one in the north-western/ north-eastern parts and plateau regions of India. So, in this study breeding for virus resistance, seventy-one exotic potato germplasm were evaluated for the host-resistance gene using molecular makers linked to the Ry genes. Germplasm were also examined for the extreme resistance to PVY by Ry gene-linked markers and confirmatory results of resistance by ELISA testing. Among all, 26 germplasm (group I) showed the presence of Ryadg gene as revealed by SCAR (RYSC3) and CAPS (ADG2/BbvI) markers and also exhibited resistance to PVY by ELISA testing after mechanical inoculation of PVYO isolate. This group I germplasm appear to be future research materials for the introgression of resistant gene Ryadg derived from S. tuberosum spp. andigena using marker-assisted selection (MAS) in the Indian potato breeding. In addition, 45 germplasm (group II) did not amplify any of the markers of Ry genes available to date derived from wild Solanum species and their resistance/susceptibility is also not known. Thus, these research findings highlighted the use of the Ryadg gene-based molecular markers to accelerate the potato breeding through MAS.

How to Cite

Tiwari, J. K., ., P., Chakrabarti, S., Kumar, V., Gopal, J., Singh, B., … Pattanayak, D. (2013). Identification of host gene conferring resistance to Potato virus Y using Ry gene-based molecular markers. Indian Journal of Horticulture, 70(03), 373–377. Retrieved from https://journal.iahs.org.in/index.php/ijh/article/view/2094

Downloads

Download data is not yet available.

Similar Articles

1 2 3 4 5 6 7 8 9 10 > >> 

You may also start an advanced similarity search for this article.

Most read articles by the same author(s)

1 2 > >>