Development of simple sequence repeats (SSRs) markers for identification of wild species and somatic hybrids of potato

Published

2020-03-25

DOI:

https://doi.org/10.5958/0974-0112.2020.00011.0

Keywords:

Solanum tuberosum, Solanum species, wild, molecular characterization.
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Authors

  • Jagesh Kumar Tiwari ICAR-Central Potato Research Institute, Shimla 171001, Himachal Pradesh
  • Rasna Zinta ICAR-Central Potato Research Institute, Shimla 171001, Himachal Pradesh
  • Vinod Kumar ICAR-Central Potato Research Institute, Shimla 171001, Himachal Pradesh
  • JiRajesh K. Singhnal ICAR-Central Potato Research Institute, Shimla 171001, Himachal Pradesh
  • Shashi Rawat ICAR-Central Potato Research Institute, Shimla 171001, Himachal Pradesh
  • SK Chakrabarti ICAR-Central Potato Research Institute, Shimla 171001, Himachal Pradesh

Abstract

SSR markers were analysed to reveal allelic variations in wild species and interspecific somatic hybrids of potato. SSR allelic profiles showed high polymorphism and distinctness in the samples. A total of 131 alleles of 13 polymorphic SSR loci were scored in 48 samples of wild species and somatic hybrids of potato. Alleles per locus ranged from 6 (STM5127/ STM5114/ STM1053/ STI0030) to 20 (STM1052) whereas PIC value varied between 0.81 (STM5127/ STM5114) to 0.94 (STM1052) in the samples. This study suggests that SSR-based genotyping and development SSR markers dataset would strengthen their utilization in identification and molecular characterization of wild species and somatic hybrids of potato.

How to Cite

Tiwari, J. K. ., Zinta, R., Kumar, V., Singhnal, J. K., Rawat, S. ., & Chakrabarti, S. . (2020). Development of simple sequence repeats (SSRs) markers for identification of wild species and somatic hybrids of potato. Indian Journal of Horticulture, 77(01), 112–115. https://doi.org/10.5958/0974-0112.2020.00011.0

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