Response of some wild species of tomato against Peanut bud necrosis virus under open-field conditions

Abstract

Thrips-borne Tospovirus pathogens adversely affect many globally important crops. Among 16 distinct virus species in the Tospovirus genus, four species including Peanut bud necrosis virus (PBNV) causing necrosis disease in tomato have been reported in India. Identification of stable sources and further utilization of wild relatives as gene sources to increase levels and diversify the bases of resistance may offer good management for the disease. A total of 13 wild species of tomato (Solanum peruvianum), two S. pimpinellifolium, one S. chilense, one S. pennellii and three check cultivars (S. lycopersicum) along with two cultivars (S. lycopersicum) having the Sw-5 and Sw-7 genes were evaluated under field conditions during three consecutive seasons (June to October 2008, July to December 2009, August 2010 to February 2011). Among all, a high degree of field resistance (>80%) was detected in seven lines of S. peruvianum (L00735, L00671, L00887, L06138), S. chilense (TL02226) and S. pimpinellifolium (L03708, TL02213) lines. The field data was also supported by negative reaction against a polyclonal antiserum of the nucleocapsid protein (N) of PBNV in direct antigen coating-enzyme linked immunosorbent assay (DAC-ELISA). The cultivars with Sw-5 and Sw-7 genes were highly susceptible to PBNV.