Effect of explants and media on plant regeneration in different tomato genotypes

Abstract

The explants were cultured on Murashige and Skoog (1962) medium supplemented with different concentrations of BAP and kinetin in combination with IAA and IBA 0.2 mg/l. Two media, i.e., MS basal medium fortified with BAP 2.5 mg/l + IAA 0.2 mg/l and kinetin 2.5 mg/l + IAA 0.2 mg/l showed the highest number of shoot formation and number of shoots per explant among 24 different media combinations tested. The cotyledon explant was found to be more efficient in shoot formation. The MS basal medium containing BAP 2.5 mg/l + IAA 0.2 mg/l for hypocotyl and MS basal medium supplemented with kinetin 2.5 mg/l + IAA 0.2 mg/l for cotyledon were found the best for days to shoot formation, percent shoot formation and number of shoots per explant. The regenerated shoots produced roots (100%) in both the cultivars Hisar Lalima and Hisar Anmol on MS medium containing 0.2 mg/l IAA and were successfully hardened and transferred to the screen-house.