Studies on in vitro propagation of sweet cherry cv. Bigarreau Noir Grossa
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Axillary shoot proliferation, in vitro propagation, sweet cherry.Issue
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Abstract
Present investigation on sweet cherry (Prunus avium L.) cv. Bigarreau Noir Grossa was carried out on its micropropagation. Surface sterilisation of shoot tip explant with 0.1% HgCl2for 10 min. yielded maximum aseptic cultures and explant survival. Using explants from forced stock stem plant cuttings significantly improved culture initiation. Maximum explant establishment (50.57%) was observed on Murashige and Skoog (1962) medium supplemented with BAP + kinetin (0.25 + 0.25 mg l-1). Treatment BAP + kinetin (0.25 + 0.25 mg l-1) accounted for maximum proliferating cultures (99.96%) with highest multiplication efficiency in terms of proliferation grade (4.0) and shoots/explant (17.28). Rooting was carried out by incubation of micro-shoots in IBA supplemented MS medium for 10 days under darkness followed by their transfer on to hormone-free MS medium with incubation under light conditions. Micro-shoots equal to or greater than 10 mm in length gave good rooting. Auxin IBA (2.50 mg l-1) was found most effective, which not only gave the highest rooting (86.33%) but also maximised root number/shoot (4.90) and root length (44.33 mm).
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