In vitro propagation of a self-incompatible cabbage line ‘Sel. 5’

Abstract

A viable protocol was developed for in vitro maintenance and multiplication of cabbage SI line ‘Selection-5’ with very strong S-allele interaction. Different types of explants, viz., apical bud, axillary bud and basal shoot sprout were tested for their in vitro regeneration ability. The morphogenetic potential varied among the explants. Apical bud proved to be the most potent explant for initial culture establishment followed by axillary bud. Murashige and Skoog (1962) medium supplemented with 2 mg l-1 BA, 0.5 mg l-1 NAA and 0.1 mg l-1 GA3 was optimum for culture establishment. The maximum in vitro shoot proliferation (5.89 ± 0.38) was obtained on MS medium supplemented with 5 mg l-1 KIN + 0.1 mg l-1 NAA + 0.1 mg l-1 GA3. The proliferation rate was significantly influenced by type and concentration of cytokinins with kinetin being more effective than BA. Half-strength MS medium supplemented with 1.0 mg l-1 IBA was most effective for rooting. The tissue cultured plants were successfully hardened and transferred to field conditions with a survival rate of 76.67 per cent.