Standardization of an efficient protocol for in vitro mass multiplication of Hybrid Tea rose cv. Raktima
Downloads
Published
Keywords:
HT rose, micropropagation, in vitro regeneration, Raktima.Issue
Section
License
Copyright (c) 2013 Indian Journal of Horticulture
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Abstract
An efficient protocol for in vitro micropropagation of hybrid tea rose cv. Raktima was standardised using axillary bud explant. Before inoculation, explants were treated with different concentrations of fungicides and bactericides. Out of different pre-treatments tried, the highest explant survival (73.83%), bud sprouting (69.28%) with minimum microbial contamination (17.16%) were obtained with carbendazim (0.2%) + dithane M-45 (0.2%) + 8-HQC (200 mg/l) for 3 h treatment on a horizontal shaker (120 rpm). The effect of different combinations of BAP (2.5, 3.0 and 3.5 mg/l), NAA (0.1 and 0.2 mg/l), and GA3 (0.3 and 0.5 mg/l) on culture establishment and shoot proliferation was studied. Murashige and Skoog (1962) medium supplemented with BAP (3.0 mg/l) + NAA (0.1 mg/l) + GA3 (0.5 mg/l) was found most effective for culture establishment with highest explant survival (94.83%) and bud sprouting (91.90%) and on shoot proliferation with highest number of micro-shoots per explant (9.50). Better rooting on micro-shoots was induced on half-strength MS basal medium supplemented with NAA (0.5 mg/l) + IBA (0.5 mg/l) with highest rooting (94.13%). The regenerated plantlets were hardened in glass jars filled with vermiculite + agropeat (1:2) moistened with one quarter-strength MS salts (macro and micro) and covered with polypropyline lids resulting in maximum survival (91.86%). After hardening, plants were successfully transferred to the glasshouse with good survival.
How to Cite
Downloads
Similar Articles
- Rucku Gupta, V K Wali, Parshant Bakshi, Mahital Jamwal, Pramod Kumar, Rafiq Ahmed, Assessment of callus induction and plant regeneration potential of leaf explants derived from in vitro and in vivo shoot of strawberry , Indian Journal of Horticulture: Vol. 77 No. 01 (2020): Indian Journal of Horticulture
- Kalu Ram, Vasugi C, Pious Thomas, Dinesh M.R, Ex vitro rooting of micro-propagated intergeneric papaya through phloroglucinol , Indian Journal of Horticulture: Vol. 80 No. 04 (2023): Indian Journal of Horticulture
- Gokulan D, Krishnan, V, Nadaradjan, S, Umamaheswari, D, Vengadessan, V., Index based selection of treatments and genotypes for the in-vitro shoot regeneration in banana , Indian Journal of Horticulture: Vol. 80 No. 1 (2023): Indian Journal of Horticulture
- J.K. Ranjan, S.K. Singh, A.K. Chakrabarti, Pragya ., In vitro shoot regeneration from cotyledonary leaf explant in chilli and bio-hardening of plantlets , Indian Journal of Horticulture: Vol. 67 No. 01 (2010): Indian Journal of Horticulture
- Mohinder Kaur, Ajmer S. Dhatt, Jagdeep S. Sandhu, Satbir S. Gosal, In vitro plant regeneration in brinjal from cultured seedling explants , Indian Journal of Horticulture: Vol. 68 No. 01 (2011): Indian Journal of Horticulture
- Poonam Chaudhary, Pritam Kalia, Raghavendra Aminedi, Shilpi Bansal, Sanjeev Kumar, Optimization of in vitro conditions favourable for effective regeneration in Pusa Meghna Indian cauliflower , Indian Journal of Horticulture: Vol. 77 No. 01 (2020): Indian Journal of Horticulture
- Shyama Kumari, Kanwar Pal Singh, T. Janakiram, D.V.S. Raju, Standardization of in vitro mass multiplication protocol for hybrid tea rose cv. Grand Gala , Indian Journal of Horticulture: Vol. 70 No. 04 (2013): Indian Journal of Horticulture
- Nandeesh Ganiga, Reeta Bhatia, Kavita Dubey, Rashmi Arora, K. P. Singh, Sapna Panwar, Haritha Bollinedi, Sarika ., Protocol for in vitro regeneration and rapid mass multiplication of apetalous male sterile lines of marigold , Indian Journal of Horticulture: Vol. 80 No. 1 (2023): Indian Journal of Horticulture
- Kanika Sharma, Manisha Thakur, Vishal Sharma, Cost effective in vitro propagation of Gisela 5 cherry rootstock , Indian Journal of Horticulture: Vol. 77 No. 04 (2020): Indian Journal of Horticulture
- P.C. Tripathi, G. Karunakaran, T. Sakthivel, Performance of litchi cultivars for off-season production under Coorg conditions of Karnataka , Indian Journal of Horticulture: Vol. 74 No. 01 (2017): Indian Journal of Horticulture
You may also start an advanced similarity search for this article.
Most read articles by the same author(s)
- Chaithra T.S, Kanhaiya Singh, Jai Prakash, S.K. Singh, Zakir Hussain, N.C. Gupta, R.R. Kumar, Development of sex-linked PCR markers for gender identification in papaya , Indian Journal of Horticulture: Vol. 81 No. 01 (2024): Indian Journal of Horticulture
- Narayan Lal, Awtar Singh, S.K. Singh, Abhay Kumar, S.D. Pandey, Vishal Nath, Morphological diversity in litchi based on phenological traits , Indian Journal of Horticulture: Vol. 80 No. 1 (2023): Indian Journal of Horticulture
- Reena Prusty, O. P. Awasthi, S.K. Singh, C. Bharadwaj, Indirect somatic embryogenesis in sweet orange cv. “Mosambi” , Indian Journal of Horticulture: Vol. 80 No. 1 (2023): Indian Journal of Horticulture
- Aparna Veluru, M Neema, S Jayasekhar, Vandita Kumari, V Selvamani, Sapna Panwar, Namita ., Kanwar Pal Singh, P Subramanian, Ravi Bhat, Evaluation of indigenous rose varieties as intercrop under coconut plantation in India , Indian Journal of Horticulture: Vol. 78 No. 03 (2021): Indian Journal of Horticulture
- Dharminder Singh, Madhu Bala, Aman Sharma, Induced mutations in chrysanthemum through gamma rays , Indian Journal of Horticulture: Vol. 80 No. 03 (2023): Indian Journal of Horticulture
- Madhumita Mallick, A.K. Dubey, S.K. Singh, R.M. Sharma, Tree morphology, yield and fruit quality of grapefruit cultivars on different rootstocks in Inceptisol , Indian Journal of Horticulture: Vol. 76 No. 03 (2019): Indian Journal of Horticulture
- Kamlesh Kumar, Manish Srivastav, S.K. Singh, Ankit Singh, Nimisha Sharma, Studies on extent of polyembryony in salt tolerant mango rootstocks , Indian Journal of Horticulture: Vol. 75 No. 01 (2018): Indian Journal of Horticulture
- Anjali Soni, Jai Prakash, S.K. Singh, A.K. Goswami, N.C. Gupta, A.K. Singh, Efficacy of gene-based markers associated with sex expression in papaya , Indian Journal of Horticulture: Vol. 74 No. 03 (2017): Indian Journal of Horticulture
- Meenu Kumari, S.K. Singh, Kishan Swaroop, Deeba Kamil, K.P. Singh, Diversity analysis of Fusarium oxysporum f. sp. gladioli isolates using Fatty Acid Methyl Ester (FAME) profiling , Indian Journal of Horticulture: Vol. 75 No. 03 (2018): Indian Journal of Horticulture
- Omem Tamut, Kanwar Pal Singh, Archana P. Raina, Namita ., Sapna Panwar, Prabhat Kumar, Pavnesh Kumar Verma, Composition and yield variation of essential oils from French marigold (Tagetes patula L.) genotypes using GC-MS , Indian Journal of Horticulture: Vol. 74 No. 01 (2017): Indian Journal of Horticulture