Conservation of medicinal yam in vitro: Effect of ionic strength, sucrose, mannitol, ABA and low temperature
Downloads
Published
DOI:
https://doi.org/10.5958/0974-0112.2019.00110.5Keywords:
Dioscorea floribunda, in vitro conservation, in vitro tuberization, low temperature.Issue
Section
License
Copyright (c) 2019 Indian Journal of Horticulture
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Abstract
The effects of ionic strength, sucrose, ABA, mannitol and low temperature was investigated on the development of slow growth cultures for in vitro conservation of medicinal yam germplasm. Though higher concentration of mannitol (5 %), lower concentration of ionic strength (1/4 MS+ 0.5 % sucrose) and sucrose (0.5 % and 1%) induced significantly high growth reduction in the plantlets, however, these treatments except sucrose not only negatively affected survival of the cultures but also abnormal leaf morphology and multiple shoot formation was observed with increase in concentration of mannitol. Sucrose (0.5 % and 1 %) not only induced slow growth but also reduced the shoot production and showed no effect on survival of cultures even maintained up to 180 days without subculturing. Storage of culture in normal MS media at low temperature (18±1°C) maintained upto 150 days not only reduced the growth parameters but also induced development of both basal and aerial microtubers. Use of low temperature could be the best option for monocrop repositories of yam in vitro. However, reduced dose of sucrose can be a suitable and economically sustainable method for in vitro conservation of D. floribunda at 25±2°C.
How to Cite
Downloads
Similar Articles
- J.S. Bisen, A.K. Singh, Impact of inorganic to organic cultivation practices on yield of tea in Darjeeling hills - A case study , Indian Journal of Horticulture: Vol. 69 No. 02 (2012): Indian Journal of Horticulture
- Sangeeta Pandey, V.S. Pundhir, Mycoparasitism of potato black scurf pathogen (Rhizoctonia solani Kuhn) by biological control agents to sustain production , Indian Journal of Horticulture: Vol. 70 No. 01 (2013): Indian Journal of Horticulture
- Indu S. Sawant, G.S. Karibasappa, Kiran P. Deokar, Dinesh Shetty, Anuradha Upadhyay, Reaction of Vitis genotypes at the National Active Germplasm Site to downy mildew infection under tropical humid conditions of India , Indian Journal of Horticulture: Vol. 69 No. 02 (2012): Indian Journal of Horticulture
- Jitendra Kumar, Gyan P. Mishra, A.A. Murkute, G. Phanikumar, Pradeep K. Naik, R.B. Srivastava, Exploring genetic relationships in Artemisia species growing in trans-Himalayan cold arid desert using RAPD markers , Indian Journal of Horticulture: Vol. 69 No. 02 (2012): Indian Journal of Horticulture
- S.K. Sain, M.L. Chadha, Evaluation of improved lines of tomato for yield performance and disease resistance under open field conditions , Indian Journal of Horticulture: Vol. 69 No. 02 (2012): Indian Journal of Horticulture
- U.S. Gautam, Rajesh Singh, Neelam Tiwari, P.S. Gurjar, Ashish Kumar, Effect of integrated nutrient management in mango cv. Sunderja , Indian Journal of Horticulture: Vol. 69 No. 02 (2012): Indian Journal of Horticulture
- N.K. Meena, V.S. Nagrare, R.P. Medhi, Thrips, Dichromothrips nakahari Mound (Thysanoptera: Thripidae) infesting the orchids in India - A new report , Indian Journal of Horticulture: Vol. 68 No. 04 (2011): Indian Journal of Horticulture
- Bhawana Bisht, M.P. Singh, B.K. Srivastava, P.K. Singh, Performance of cucumber varieties in a naturally ventilated polyhouse , Indian Journal of Horticulture: Vol. 68 No. 04 (2011): Indian Journal of Horticulture
- Dinesh Kumar, D.B. Singh, Effect of fertigation on growth, yield and quality of almond under Kashmir conditions , Indian Journal of Horticulture: Vol. 75 No. 02 (2018): Indian Journal of Horticulture
- B.P. Shahi, P.K. Singh, V.K. Singh, Diwakar Singh, Triple test-cross analysis for fruit yield and some component characters in cucumber , Indian Journal of Horticulture: Vol. 68 No. 01 (2011): Indian Journal of Horticulture
<< < 33 34 35 36 37 38 39 40 41 > >>
You may also start an advanced similarity search for this article.
Most read articles by the same author(s)
- Sanghamitra Samantaray, Satyabrata Maiti, In vitro organogenesis in Aloe barbadensis Mill.: An aloin A rich plant , Indian Journal of Horticulture: Vol. 67 No. 01 (2010): Indian Journal of Horticulture