A universal system for matK gene based diagnostic markers to identify the species in Cucurbitaceae
Downloads
Published
DOI:
https://doi.org/10.5958/0974-0112.2020.00106.1Keywords:
Cucurbits, genetic diversity, Momordica, Luffa, systematics, taxonomyIssue
Section
License
Copyright (c) 2020 Indian Journal of Horticulture
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Abstract
Designing and validation of universal primer combination for matK gene in Cucurbitaceae is described. Using the universal primers already reported, matK gene amplification was attempted in 24 Momordica and 4 Luffa accessions, which was successful at varying rates. Amplified markers were gel eluted, Sanger sequenced and the sequences along with the matK sequences from other Cucurbitaceae genera available at GenBank, were aligned to identify the conserved regions in the locus. Primer combination was designed for the conserved regions and this primer set was found successful for all the Momordica and Luffa accessions. Subsequently, the primer combination was shown successful for other genera such as Benincasa, Cucumis, Cucurbita, Citrullus, Coccinia, Trichosanthes and Lagenaria, suggesting it as a universal system for matK gene in Cucurbitaceae family.
How to Cite
Downloads
Similar Articles
- Tashi Dolma, A.J. Gupta, N. Ahmed, Variability, heritability and genetic advance in lettuce , Indian Journal of Horticulture: Vol. 67 No. Special Issue (2010): Indian Journal of Horticulture
- Sohnika Rani, Arti Sharma, Kiran Kour, Manish Sharma, Assessment and exploitation of genetic divergence in pecan nut , Indian Journal of Horticulture: Vol. 79 No. 3 (2022): Indian Journal of Horticulture
- Dipak Nayak, A.K. Singh, Manish Srivastav, Estimation of genetic parameters of fruit quality traits in mango hybrid population , Indian Journal of Horticulture: Vol. 70 No. 01 (2013): Indian Journal of Horticulture
- X.L. Li, J.K. Zhang, M.J. Li, B.B. Zhou, Q. Zhang, Q.P. Wei, Influence of six dwarfing interstocks on the ‘Fuji’ apple under drought stress , Indian Journal of Horticulture: Vol. 74 No. 03 (2017): Indian Journal of Horticulture
- Bolagam Ravikumar, K. K Dhatt, Assessment of genetic parameters in periwinkle through diallel analysis , Indian Journal of Horticulture: Vol. 80 No. 03 (2023): Indian Journal of Horticulture
- S.S. Dey, T.K. Behera, A.D. Munshi, Anand Pal, Combining ability analysis in bitter gourd , Indian Journal of Horticulture: Vol. 67 No. 03 (2010): Indian Journal of Horticulture
- B.R. Choudhary, Sudhakar Pandey, P.K. Singh, V. Pandey, Genetic diversity analysis for quantitative traits in hermaphrodite ridge gourd [Luffa acutangula (Roxb.) L.] , Indian Journal of Horticulture: Vol. 71 No. 02 (2014): Indian Journal of Horticulture
- Israr Ahmad, S. Bhagat, T.V.R.S. Sharma, Krishna Kumar, P. Simachalam, R.C. Srivastava, ISSR and RAPD marker based DNA fingerprinting and diversity assessment of Annona spp. in South Andamans , Indian Journal of Horticulture: Vol. 67 No. 02 (2010): Indian Journal of Horticulture
- Dimpy Raina, W.S. Dhillon, Kuldeep Singh, Analysis of genetic diversity in pear germplasm using morphological traits and DNA markers , Indian Journal of Horticulture: Vol. 68 No. 03 (2011): Indian Journal of Horticulture
- P.L. Saran, A.K. Godara, S.K. Sehrawat, Morphological variability among low chilling peach genotypes under Dehradun conditions , Indian Journal of Horticulture: Vol. 67 No. 01 (2010): Indian Journal of Horticulture
<< < 3 4 5 6 7 8 9 10 11 12 > >>
You may also start an advanced similarity search for this article.
Most read articles by the same author(s)
- T. Pradeepkumar, Minnu, A.J, Reshmika P.K., K. Veni, R.C. Varun, Deepu Mathew, Characterization and maintenance of promising gynoecious bitter gourd line through hormonal regulation and micropropagation , Indian Journal of Horticulture: Vol. 79 No. 3 (2022): Indian Journal of Horticulture
- Anju Viswanath, Deepu Mathew, Shylaja M.R., Candidate markers assay for Capsicum pungency , Indian Journal of Horticulture: Vol. 76 No. 03 (2019): Indian Journal of Horticulture