Influence of explant collection period, antibrowning strategy and growth regulators composition on in vitro propagation of Bhagwa pomegranate
Downloads
Published
DOI:
https://doi.org/10.5958/0974-0112.2019.00042.2Keywords:
Punica granatum, explant establishment, In vitro, plantlet regenerationIssue
Section
License
Copyright (c) 2019 Indian Journal of Horticulture
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Abstract
In vitro propagation is a rapid mean for production of disease-free and healthy plants. Using nodal segments for in vitro propagation of pomegranate cv. Bhagwa, the effect of time of explant collection and anti-browning strategy on culture establishment were investigated. A combination of growth regulators (BAP, NAA) and growth substances (AgNO3, Adenine sulphate) were also tested for shoot bud initiation, shoot proliferation and in vitro rooting. In a culture cycle of April to July months, the maximum explant establishment (58.07%) was recorded in the month of April. The medium supplemented with activated charcoal (2.5 g l-1) induced significantly higher culture establishment (63.9%) than control and the treatments involving pre-culture shaking of explants with polyvinylpyrrolidone (PVP) (1.0%) solution or antioxidants (100 mg l-1 citric acid + 150 mg l-1 ascorbic acid) solution. For shoot bud initiation from nodal segments, different levels of BAP (0.5, 1.0, 1.5 and 2.0 mg l-1) alone or with NAA (0.25 mg l‑1) were tested. The significantly highest response (85.17%) was achieved on MS medium supplemented with BAP (1.0 mg l-1) + NAA (0.25 mg l-1). The AgNO3 (1.5 mg l-1) enabled maximum survival of cultured shoots during sub-culture stage. The MS medium containing BAP (0.5 mg l-1) + NAA (0.5 mg l-1) + AgNO3 (1.5 mg l-1) + Adenine sulphate (40 mg l-1) was good for shoot multiplication. The half strength MS medium supplemented with NAA (1.0 mg l-1), was suitable for rooting of shoots in vitro
How to Cite
Downloads
Similar Articles
- J.K. Ranjan, S.K. Singh, A.K. Chakrabarti, Pragya ., In vitro shoot regeneration from cotyledonary leaf explant in chilli and bio-hardening of plantlets , Indian Journal of Horticulture: Vol. 67 No. 01 (2010): Indian Journal of Horticulture
- Hare Krishna, S.K. Singh, R.K. Sairam, S.K. Verma, Effect of different factors on in vitro shoot tip culture establishment in mango , Indian Journal of Horticulture: Vol. 67 No. 03 (2010): Indian Journal of Horticulture
- Nripendra V. Singh, S.K. Singh, V.B. Patel, In vitro culture establishment studies on pomegranate , Indian Journal of Horticulture: Vol. 68 No. 03 (2011): Indian Journal of Horticulture
- Hira Singh, T.S Dhillon, In vitro morphogenesis and plant regeneration response from hot pepper (Capsicum annuum L.) cultivar Punjab Guchhedar , Indian Journal of Horticulture: Vol. 71 No. 03 (2014): Indian Journal of Horticulture
- Mohinder Kaur, Ajmer S. Dhatt, Jagdeep S. Sandhu, Satbir S. Gosal, In vitro plant regeneration in brinjal from cultured seedling explants , Indian Journal of Horticulture: Vol. 68 No. 01 (2011): Indian Journal of Horticulture
- Nandeesh Ganiga, Reeta Bhatia, Kavita Dubey, Rashmi Arora, K. P. Singh, Sapna Panwar, Haritha Bollinedi, Sarika ., Protocol for in vitro regeneration and rapid mass multiplication of apetalous male sterile lines of marigold , Indian Journal of Horticulture: Vol. 80 No. 1 (2023): Indian Journal of Horticulture
- Rucku Gupta, V K Wali, Parshant Bakshi, Mahital Jamwal, Pramod Kumar, Rafiq Ahmed, Assessment of callus induction and plant regeneration potential of leaf explants derived from in vitro and in vivo shoot of strawberry , Indian Journal of Horticulture: Vol. 77 No. 01 (2020): Indian Journal of Horticulture
- Gokulan D, Krishnan, V, Nadaradjan, S, Umamaheswari, D, Vengadessan, V., Index based selection of treatments and genotypes for the in-vitro shoot regeneration in banana , Indian Journal of Horticulture: Vol. 80 No. 1 (2023): Indian Journal of Horticulture
- Poonam Chaudhary, Pritam Kalia, Raghavendra Aminedi, Shilpi Bansal, Sanjeev Kumar, Optimization of in vitro conditions favourable for effective regeneration in Pusa Meghna Indian cauliflower , Indian Journal of Horticulture: Vol. 77 No. 01 (2020): Indian Journal of Horticulture
- Kalu Ram, Vasugi C, Pious Thomas, Dinesh M.R, Ex vitro rooting of micro-propagated intergeneric papaya through phloroglucinol , Indian Journal of Horticulture: Vol. 80 No. 04 (2023): Indian Journal of Horticulture
You may also start an advanced similarity search for this article.
Most read articles by the same author(s)
- Harsimran Kamboj, Krishan Kumar, J. S. Brar, Anil Kumar, P. K Arora, Optimization of in vitro propagation of jamun variety Konkan Bahadoli , Indian Journal of Horticulture: Vol. 80 No. 03 (2023): Indian Journal of Horticulture
- Harsimran Singh, Anita Arora, Krishan Kumar, Preliminary screening of citrus hybrids for identifying tolerance to Phytophthora nicotianae , Indian Journal of Horticulture: Vol. 81 No. 01 (2024): Indian Journal of Horticulture
- Anil Kumar, Shashi Pathania, P.K. Arora, Rootstock evaluation for sweet orange cv. Early Gold in arid irrigated region of Punjab , Indian Journal of Horticulture: Vol. 75 No. 01 (2018): Indian Journal of Horticulture
- Mandeep Pathania, Jagdish Kumar Arora, Parshotam Kumar Arora, Incidence and severity of insect-pests and diseases of Kinnow mandarin , Indian Journal of Horticulture: Vol. 78 No. 03 (2021): Indian Journal of Horticulture
- Arun Kishor, Raj Narayan, Manoj Brijwal, Brij Lal Attri, Anil Kumar, Sovan Debnath, Storage behaviour of apple cultivars under ambient conditions , Indian Journal of Horticulture: Vol. 75 No. 02 (2018): Indian Journal of Horticulture
- Anil Kumar, Navin Singh, K.K. Misra, Vishal Nirgude, Effect of foliar spray of calcium chloride and boric acid on shelf-life of guava , Indian Journal of Horticulture: Vol. 74 No. 04 (2017): Indian Journal of Horticulture
- S.S. Manhas, B.S. Gill, Sushil Sharma, Krishan Kumar, Effect of different planting material, planting dates and harvesting dates on growth, yield and quality of turmeric , Indian Journal of Horticulture: Vol. 68 No. 02 (2011): Indian Journal of Horticulture
- B. L. Attri, M. S. Mer, Arun Kishor, Anil Kumar, Shelf life and quality of apple at ambient conditions affected by different concentrations of Aloe vera gel and neem oil , Indian Journal of Horticulture: Vol. 75 No. 04 (2018): Indian Journal of Horticulture
- A. Kishor, Raj Narayan, Manoj Brijwal, B.L. Attri, Anil Kumar, Sovan Debnath, Performance of some new apple cultivars for yield and physico-chemical characters under mid-hill conditions of Uttarakhand , Indian Journal of Horticulture: Vol. 75 No. 01 (2018): Indian Journal of Horticulture
- Anil Kumar, Shashi Pathania, Parshotam Kumar Arora, Performance of early ripened varieties of ‘Sweet Orange’ in arid irrigated region of Punjab , Indian Journal of Horticulture: Vol. 77 No. 01 (2020): Indian Journal of Horticulture