Characterization and maintenance of promising gynoecious bitter gourd line through hormonal regulation and micropropagation

Downloads

Published

2022-09-30

Keywords:

Momordica charantia L., sex expression, micropropagation, genetics of gynoecy
Dimensions Badge

Issue

Vol. 79 No. 3 (2022): Indian Journal of Horticulture

Section

Research Articles

License

Creative Commons License

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Authors

  • T. Pradeepkumar Department of Vegetable Science, College of Agriculture, Kerala Agricultural University, Thrissur–680656, Kerala, India
  • Minnu, A.J Department of Vegetable Science, College of Agriculture, Kerala Agricultural University, Thrissur–680656, Kerala, India
  • Reshmika P.K. Department of Vegetable Science, College of Agriculture, Kerala Agricultural University, Thrissur–680656, Kerala, India
  • K. Veni Department of Vegetable Science, College of Agriculture, Kerala Agricultural University, Thrissur–680656, Kerala, India
  • R.C. Varun Department of Vegetable Science, College of Agriculture, Kerala Agricultural University, Thrissur–680656, Kerala, India
  • Deepu Mathew Centre for Plant Biotechnology and Molecular Biology, College of Agriculture, Kerala Agricultural University, Thrissur, Kerala–680656, India

Abstract

New gynoecious bitter gourd line, KAU-MCGy-101 identified from Kerala Agricultural University, India, was morphologically characterized. Silver thiosulphate (STS) was used for altering the sex expression of gynoecious line. STS induced hermaphrodite flowers in gynoecious plants and maximum number of hermaphrodite flowers was in plants receiving single spray at 200 ppm after emergence of first female flower. F1 was generated by crossing with monoecious variety Preethi and all the hybrids were monoecious in nature. Expression of gynoecious character in the sib-mated gynoecious inbred and monoecy in F1 hybrids, indicate the recessive nature of gynoecy in bitter gourd. Gynoecious inbred was also maintained through micropropagation. The shoot tip explants cultured on MS medium supplemented with 2.0 mgL-1 BA followed by 1.5 mgL-1 BA have given the highest shoot initiation response in 20.75 days. Multiple shoots, up to 5.50 per explant, were induced on MS medium with 2.0 mgL-1 BA and microshoots elongated best with 0.5 mgL-1 IAA + 0.5 mgL-1 NAA. Best in vitro rooting was on half strength MS medium with 3.0 gL-1 activated charcoal and 1.0 mgL-1 IBA. The gynoecious expression was found to be stable in tissue culture regenerated progenies.

How to Cite

Pradeepkumar, T., A.J, M., P.K., R., Veni, K., Varun, R., & Mathew, D. (2022). Characterization and maintenance of promising gynoecious bitter gourd line through hormonal regulation and micropropagation. Indian Journal of Horticulture, 79(3), 287–295. Retrieved from https://journal.iahs.org.in/index.php/ijh/article/view/491

Downloads

Download data is not yet available.

References

Agarwal, M. and Kamal, R. 2004. In vitro clonal

propagation of Momordica charantia L. Indian J.

Biotechnol. 3: 426-30.

Al Munsur, M.A.Z., Haque, M.S., Nasiruddin,

K.M. and Hossain, M.S. 2009. In vitro

propagation of bitter gourd (Momordica

charantia L.) from nodal and root segments.

Plant Tiss. Cult. Biotechnol. 19: 45-52.

Behera, T.K., Dey, S.S. and Sirohi, P.S. 2006.

DBGy-201 and DBGy-202: two gynoecious

lines in bitter gourd (Momordica charantia L.)

isolated from indigenous source. Indian J.

Genet. Plant Breed. 66: 61-62.

Behera, T.K., Dey, S.S., Munshi, A.D., Gaikwad,

A.B., Pal, A. and Singh, I. 2009. Sex inheritance

and development of gynoecious hybrids in bitter

gourd (Momordica charantia L.). Sci. Hortic.

: 130-33.

Devendra, N.K., Subhash, B. and Seetharam,

Y.N. 2009. Callus growth and plant regeneration

in Momordica dioica (Roxb.) Willd.,

Cucurbitaceae. Am. Eurasian J. Sustain. Agric.

:743-48

Fridborg, G., Pedersen, M., Landström, L.E.

and Eriksson, T. 1978. The effect of activated

charcoal on tissue cultures: adsorption of

metabolites inhibiting morphogenesis. Physiol.

Plant. 43:104-106.

Friedlander, M., Atsmon, D. and Galun, E. 1977.

Sexual differentiation in cucumber: The effects

of abscisic acid and other growth regulators on

various sex genotypes. Plant Cell Physiol. 18:

-69.

Gantait, S., Mandal, N. and Das, P.K. 2009.

Impact of auxins and activated charcoal on in

vitro rooting of Dendrobium chrysotoxum Lindl.

cv. Golden Boy. J. Trop. Agric. 47: 84-86.

Gaspar, T., Kevers, C., Penel, C., Greppin, H.,

Reid, D.M. and Thorpe, T.A. 1996. Plant hormones

and plant growth regulators in plant tissue culture.

In vitro Cell. Dev. Biol. Plant. 32: 272-89.

Liu, R.B., Gupta, S.K., Liaw, S.Y., Chien, C.H.

and Tsay, H.S. 2011. In vitro studies on newly

developed variety of Momordica charantia L.

Hualien No. 1 and its metabolites analysis. Int. J.

Integr. Biol. 11: 155.

Mishra, S., Behera, T.K. and Munshi, A.D. 2015.

Induction and morphological characterization

of hermaphrodite flowers in a gynoecious line

of bitter gourd by silver nitrate, gibberellic

acid, and silver thiosulfate. Int. J. Veg. Sci. 21:

-11.

Mishra, S., Behera, T.K., Munshi, A.D.,

Bharadwaj, C. and Rao, A.R. 2015. Inheritance

of gynoecism and genetics of yield and yield

contributing traits through generation mean

analysis in bitter gourd. Indian J. Hortic. 72:

-22.

More, T.A. and Sheshadri, V.S. 1998. Sex

expression and sex modification. In: Cucurbits;

Nayar NM, More TA (Eds). Oxford and IBH

Publishing Co. Pvt. Ltd., New Delhi, pp.39-66.

Murashige, T. and Skoog, F. 1962. A revised

medium for rapid growth and bioassay with

tobacco tissue cultures. Physiol. Plant. 15:

-97.

National Bureau of Plant Genetic Resources.

Minimal Descriptors of Agri-Horticultural

Crops, Part II: Vegetable Crops. New Delhi,

pp. 262.

Pradeepkumar, T., Sujatha, R., Krishnaprasad,

B.T. and Johnkutty, I. 2007. New source of

male sterility in ridge gourd (Luffa acutangula

(L.) Roxb.) and its maintenance through in vitro

culture. CGC Report 30: 60-63.

Ram, D., Kumar, S., Banerjee, M.K. and Kalloo, G.

Occurrence, identification and preliminary

characterisation of gynoecism in bitter gourd

(Momordica charantia L.). Indian J. Agric. Sci.

: 348-349.

Ram, D., Kumar, S., Banerjee, M.K., Singh, B.

and Singh, S. 2002. Developing bitter gourd

(Momordica charantia L.) populations with a very

high proportion of pistillate flowers. CGC Report

: 65-66.

Ram, D., Kumar, S., Singh, M., Rai, M. and

Kalloo, G. 2006. Inheritance of gynoecism

in bitter gourd (Momordica charantia L.). J.

Heredity 97: 294-95.

Rao, G.P., Behera, T.K., Munshi, A.D. and Dev,

B. 2017. Estimation of genetic components of

variation and heterosis studies in bitter gourd for

horticultural traits. Indian J. Hortic. 74: 227-32.

Saha, S. and Behera, T.K. 2015. Standardization

of techniques for in vitro multiplication of

gynoecious line in bitter gourd. Vegetos 28:

-53.

Song, L. and Gao, F. 2006. Changes of

endogenous hormones in Momordica charantia

during in vitro culture. Chin. Bull. Bot. 23:

-96.

Vasudevan, A., Selvaraj, N., Kumar, P.S and

Ganapathi, A. 2001. Multiple shoot induction

from shoot tip explants of Cucumber (Cucumis

sativus L.). CGC Report 24: 8-12.

Zhao, X.C., Qu, X., Mathews, D.E. and

Schaller, G.E. 2002. Effect of ethylene pathway

mutations upon expression of the ethylene

receptor ETR1 from Arabidopsis. Plant Physiol. 130: 1983-91.

Similar Articles

<< < 1 2 3 4 5 6 7 8 9 10 > >> 

You may also start an advanced similarity search for this article.