Characterization of acid lime genotypes using SSR markers

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Published

2022-12-19

Keywords:

Citrus aurantifolia Swingle, genetic diversity, SSR markers, polymorphism, primers.
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Issue

Vol. 79 No. 4 (2022): Indian Journal of Horticulture

Section

Research Articles

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This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Authors

  • Shilpy Kumari Division of Fruit Science, Faculty of Agriculture, SKUAST-J, Chatha, Jammu 180009, JKUT, India
  • Akash Sharma Advanced Centre for Horticulture Research, Udheywalla, SKUAST-Jammu 180018, JKUT, India
  • Parshant Bakshi Advanced Centre for Horticulture Research, Udheywalla, SKUAST-Jammu 180018, JKUT, India
  • Romesh Salgotra School of Biotechnology, SKUAST-J, Chatha, Jammu 180009 JKUT, India
  • Manish Sharma Division of Statistics and Computer Sciences, SKUAST-J, Chatha, Jammu 180009 JKUT, India
  • Vishal Gupta Advanced Centre for Horticulture Research, Udheywalla, SKUAST-Jammu 180018, JKUT, India
  • G.K. Rai Division of Statistics and Computer Sciences, SKUAST-J, Chatha, Jammu 180009 JKUT, India

Abstract

The determination of genetic variation at morphological and molecular level is of fundamental importance to the citrus breeders for the development of elite cultivars with desirable traits. This paper aimed to study the genetic diversity among seventy acid lime landraces using simple sequence repeat (SSR) markers. The results revealed a wide range of diversity in 70 genotypes in terms of fruit weight (30.50-56.26 g), juice (29.01-57.13%), acidity (6.18-8.35 %), and ascorbic acid (22.22-36.81 mg/100 ml juice). Twenty-five SSR markers were used to detect polymorphism among these genotypes. A total of 99 alleles were detected with an average of 4.71 alleles per locus. Polymorphism information content ranged between 0.41 (TAA15) to 0.93 (CT19) with an average of 0.73 and polymorphism ranged from 33.33 to 66.67 % with an average of 50.95 %. The markers were able to distributed the genotypes into two major clusters. The Jaccard’s genetics similarity value varied from 0.08 to 0.60. Highest genetic similarity index was observed between JMU-Sum (58) and JMU-Sun (61) (0.60). However, least similarity index was observed between JMU-Jib (36) and JMU-Balli (30) (0.08). Out of all the selected genotypes, JMU-Nag (70) and JMU-Jib (36) were found most promising genotypes, and can be suggested for use in further breeding programmes.

How to Cite

Kumari, S., Sharma, A., Bakshi, P., Salgotra, R., Sharma, M., Gupta, V., & Rai, G. (2022). Characterization of acid lime genotypes using SSR markers. Indian Journal of Horticulture, 79(4). Retrieved from https://journal.iahs.org.in/index.php/ijh/article/view/509

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