Cross-species amplification of selected SSR markers to jackfruit and its related species

Published

2019-09-10

DOI:

https://doi.org/10.5958/0974-0112.2019.00061.6

Keywords:

Artocarpus heterophyllus, transferability, microsatellite markers.
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Authors

  • Emmanuel Bonifacio S. Timog Institute of Crop Science, College of Agriculture and Food Science, University of the Philippines Los Baños, College, Laguna
  • Yosi Zendra Joni Institute of Crop Science, College of Agriculture and Food Science, University of the Philippines Los Baños, College, Laguna
  • Renerio P. Gentallan Jr Institute of Crop Science, College of Agriculture and Food Science, University of the Philippines Los Baños, College, Laguna
  • Nestor C. Altoveros Institute of Crop Science, College of Agriculture and Food Science, University of the Philippines Los Baños, College, Laguna
  • Teresita H. Borromeo Institute of Crop Science, College of Agriculture and Food Science, University of the Philippines Los Baños, College, Laguna
  • Leah E. Endonela Institute of Crop Science, College of Agriculture and Food Science, University of the Philippines Los Baños, College, Laguna
  • Antonio G. Lalusin Institute of Crop Science, College of Agriculture and Food Science, University of the Philippines Los Baños, College, Laguna

Abstract

Out of 28 SSR markers screened, seven (25%) markers were found to stable, reproducible and highly polymorphic amplifications in 16 Artocarpus heterophyllus accessions and 5 related species. The number of alleles per marker ranged from 6 to 17 with a PIC per marker ranging from 0.73 to 0.89. The haplotype also showed unique banding patterns in the 21 accessions of Artocarpus across the seven primers with 67% similarity. This denotes a potentially successful interfamilial amplification and transferability of the selected SSR markers to jackfruit.

How to Cite

Timog, E. B. S., Joni, Y. Z., Gentallan Jr, R. P., Altoveros, N. C., Borromeo, T. H., Endonela, L. E., & Lalusin, A. G. (2019). Cross-species amplification of selected SSR markers to jackfruit and its related species. Indian Journal of Horticulture, 76(03), 377–381. https://doi.org/10.5958/0974-0112.2019.00061.6

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