Characterization of acid lime genotypes using SSR markers

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Published

2022-12-19

Keywords:

Citrus aurantifolia Swingle, genetic diversity, SSR markers, polymorphism, primers.
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Issue

Vol. 79 No. 4 (2022): Indian Journal of Horticulture

Section

Research Articles

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This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Authors

  • Shilpy Kumari Division of Fruit Science, Faculty of Agriculture, SKUAST-J, Chatha, Jammu 180009, JKUT, India
  • Akash Sharma Advanced Centre for Horticulture Research, Udheywalla, SKUAST-Jammu 180018, JKUT, India
  • Parshant Bakshi Advanced Centre for Horticulture Research, Udheywalla, SKUAST-Jammu 180018, JKUT, India
  • Romesh Salgotra School of Biotechnology, SKUAST-J, Chatha, Jammu 180009 JKUT, India
  • Manish Sharma Division of Statistics and Computer Sciences, SKUAST-J, Chatha, Jammu 180009 JKUT, India
  • Vishal Gupta Advanced Centre for Horticulture Research, Udheywalla, SKUAST-Jammu 180018, JKUT, India
  • G.K. Rai Division of Statistics and Computer Sciences, SKUAST-J, Chatha, Jammu 180009 JKUT, India

Abstract

The determination of genetic variation at morphological and molecular level is of fundamental importance to the citrus breeders for the development of elite cultivars with desirable traits. This paper aimed to study the genetic diversity among seventy acid lime landraces using simple sequence repeat (SSR) markers. The results revealed a wide range of diversity in 70 genotypes in terms of fruit weight (30.50-56.26 g), juice (29.01-57.13%), acidity (6.18-8.35 %), and ascorbic acid (22.22-36.81 mg/100 ml juice). Twenty-five SSR markers were used to detect polymorphism among these genotypes. A total of 99 alleles were detected with an average of 4.71 alleles per locus. Polymorphism information content ranged between 0.41 (TAA15) to 0.93 (CT19) with an average of 0.73 and polymorphism ranged from 33.33 to 66.67 % with an average of 50.95 %. The markers were able to distributed the genotypes into two major clusters. The Jaccard’s genetics similarity value varied from 0.08 to 0.60. Highest genetic similarity index was observed between JMU-Sum (58) and JMU-Sun (61) (0.60). However, least similarity index was observed between JMU-Jib (36) and JMU-Balli (30) (0.08). Out of all the selected genotypes, JMU-Nag (70) and JMU-Jib (36) were found most promising genotypes, and can be suggested for use in further breeding programmes.

How to Cite

Kumari, S., Sharma, A., Bakshi, P., Salgotra, R., Sharma, M., Gupta, V., & Rai, G. (2022). Characterization of acid lime genotypes using SSR markers. Indian Journal of Horticulture, 79(4). Retrieved from https://journal.iahs.org.in/index.php/ijh/article/view/509

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References

Abhilash, K., Kerutagi, M.G., Nagesh, N., Satish,

D. and Manjula, K. 2017. Evaluation of the elite

strains of acid lime (Citrus aurantifolia Swingle)

for the quality parameters. Int. J. Chemical

Studies, 5: 112-17.

Anonymous. 2018. District-Wise/Kind-Wise

estimated Area under Major Horticulture Crops

J&K. Department of Horticulture, Jammu and

Kashmir.

AOAC. 1980. Offical Methods of Analysis,

Association of Offical Analytical Chemists, 13th

edition. Washington. DC. Pp. 1018.

Barkley, N.A., Roose, M.L., Krueger, R.R. and

Federici CT. 2006. Assessing genetic diversity

and population structure in a citrus germplasm

collection utilizing simple sequence repeat

markers (SSRs). Theor. Appl. Genet. 112:

-31.

Cristofani, M., Figueiredo, J.O., Penteado, M.,

Targon, N. and Machado M. A. 2003. Differentiation

of lemon varieties by microsatellites. Laranja.

Cord. 241: 165-75.

Dabbarma, N. and Hazarika, B.N. 2016. Effect

of plant growth regulators and chemicals on

yield and quality of acid lime (Citrus aurantifolia

Swingle) under foot hill condition of Arunachal

Pradesh. Int. J. Agri. Env. & Biotech. 9: 231-

Doyle, J.J. and Doyle, J.L. 1990. Isolation of

Plant DNA from fresh tissue. Focus. 12: 13-15.

Ghosh, D., Bhose, S., Manimekalai, R. and

Gowda, S. 2013. Molecular detection of

Candidatus Phytoplasma spp. causing witches’

broom disease of acid lime (Citrus aurantifolia)

in India. J. Plant. Biochem. Biotech. 22: 343-

IPGRI. 1999. Descriptors of Citrus. International

Plant Genetic Resource Institute. Rome, Italy.

Khan, M.M., Mumtaz, S., Ahmad, S., Abbas,

M. and Khan, I. A. 2008. Some studies on the

morphology of Kinnow mandarin and Feutrell’s

early. Pak. J. Agri. Sci. 45: 424-31.

Naz, S., Shahzadi, K., Rashid, S., Saleem,

F., Zafarulla. and Ahmad S. 2014. Molecular

characterization and phylogenetic relationship

of different citrus varieties of Pakistan. J. Animal

Plant Sci. 24: 315-20.

Novelli, V.M., Machado, M.A. and Lopes, C.R.

Isoenzymatic polymorphism in citrus spp.

and Poncirus trifoliata (L.) Raf. (Rutaceae).

Genet. Mol. Biol. 23: 1163-68.

Rohlf, F.J. 2000. NTSYSpc, numerical taxonomy

and multivariate analysis system, version 2.11a.

New York, Excel Software, pp, 44.

Shahnazari, N., Noormohammadi, Z., Sheidai,

M. and Koohdar, F. 2022. A new insight on

genetic diversity of sweet oranges: CAPs-SSR

and SSR markers. J. Genet Eng. Biotechnol.

: 105.

Shahzadi, K., Naz, S. and Riaz, S. 2014.

Assessing genetic diversity of Pakistani citrus

varieties using microsatellite markers. J. Animal

Plant Sci. 24: 1752-57.

Sharafi, A.A., Abkenar, A.A., Sharafi, A. and

Masaeli, M. 2016. Genetic variation assessment

of acid lime accessions collected from south of

Iran using SSR and ISSR molecular markers.

Physiol. Mol. Biol. Plants. 22: 87-95.

Shrestha, R.L., Dhakal, D.D., Gautum, D.M.,

Paudyal, K.P. and Shrestha, S. 2012. Genetic

diversity assessment of acid lime (Citrus

aurantifolia Swingle) landraces in Nepal,

using SSR markers. American J. Plant Sci. 3:

-81.

Teklewood, A. and Becker, H. 2006. Geographic

pattern of genetic diversity among 43 Ethiopian

mustard (Brassica carinata A. Braun) accession

as revealed by RAPD analysis. Genet. Resour.

Crop Evol. 53: 1173–85

Yadlod, S.S., Bhalerao, R.V. and Pingle, S.N.

Variability Studies of Strains of Kagzi Lime

(Citrus aurantifolia Swingle) in Latur District of

Maharashtra. Agri. Sci. Dig.- Research J. 38:

-51.

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