Morphological and molecular characterization of Gomphrena genotypes

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Published

2023-12-30

DOI:

https://doi.org/10.58993/ijh/2023.80.4.4

Keywords:

Gomphrena globosa L., characterization, genetic diversity, polymorphism
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Vol. 80 No. 04 (2023): Indian Journal of Horticulture

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Articles

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Copyright (c) 2023 Indian Journal of Horticulture

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This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

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Authors

  • Devarai Lava Kumar Department of Floriculture and Landscape Architecture, University of Horticultural Sciences, Bagalkot-587104, Karnataka, India.
  • B. S. Kulkarni Department of Floriculture and Landscape Architecture, University of Horticultural Sciences, Bagalkot-587104, Karnataka, India.
  • Pavan Kumar P Department of Floriculture and Landscape Architecture, University of Horticultural Sciences, Bagalkot-587104, Karnataka, India.
  • Rekha B. Chittapur Department of Floriculture and Landscape Architecture, University of Horticultural Sciences, Bagalkot-587104, Karnataka, India

Abstract

Gomphrena is one of India’s emerging traditional flower crops widely used for garland making. A comparative
investigation of morphological and molecular analysis of seventeen Gomphrena genotypes was conducted to
characterize and assess their genetic diversity for commercial horticultural traits. Morphological observations
have shown drastic variation in growth, flowering, quality and yield parameters in all seventeen genotypes.
Clustering based on D2 statistics assembled the Gomphrena genotypes into seven clusters, demonstrating
the existence of wide genetic divergence at the phenotypic level. The major cluster-I included six genotypes,
cluster-IV and VI with three genotypes, cluster-II with two genotypes and other clusters with only solo genotype
based on similar morphological traits. Further, twenty ISSR primers were used to estimate the genetic diversity
and documentation of genotypes through molecular characterization. Thirteen ISSR primers were shown
polymorphism with 93 loci across the Gomphrena genotypes. Among these 93 loci, 79 were polymorphic (86.02
%) in nature, portraying the high allelic diversity. All the primers displayed good discriminating power, as
indicated by their PIC values, with a mean value of 0.21. Molecular profiling through MegaX and PCoA analyses
also separated the Gomphrena globosa population into two clusters and placed them into two plots.

How to Cite

Kumar, D. L., Kulkarni, B. S., P, P. K., & Chittapur, R. B. (2023). Morphological and molecular characterization of Gomphrena genotypes. Indian Journal of Horticulture, 80(04), 326–332. https://doi.org/10.58993/ijh/2023.80.4.4

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