Morphological and molecular characterization of Gomphrena genotypes

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Published

2023-12-30

DOI:

https://doi.org/10.58993/ijh/2023.80.4.4

Keywords:

Gomphrena globosa L., characterization, genetic diversity, polymorphism
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Vol. 80 No. 04 (2023): Indian Journal of Horticulture

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Articles

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Copyright (c) 2023 Indian Journal of Horticulture

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This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Authors

  • Devarai Lava Kumar Department of Floriculture and Landscape Architecture, University of Horticultural Sciences, Bagalkot-587104, Karnataka, India.
  • B. S. Kulkarni Department of Floriculture and Landscape Architecture, University of Horticultural Sciences, Bagalkot-587104, Karnataka, India.
  • Pavan Kumar P Department of Floriculture and Landscape Architecture, University of Horticultural Sciences, Bagalkot-587104, Karnataka, India.
  • Rekha B. Chittapur Department of Floriculture and Landscape Architecture, University of Horticultural Sciences, Bagalkot-587104, Karnataka, India

Abstract

Gomphrena is one of India’s emerging traditional flower crops widely used for garland making. A comparative
investigation of morphological and molecular analysis of seventeen Gomphrena genotypes was conducted to
characterize and assess their genetic diversity for commercial horticultural traits. Morphological observations
have shown drastic variation in growth, flowering, quality and yield parameters in all seventeen genotypes.
Clustering based on D2 statistics assembled the Gomphrena genotypes into seven clusters, demonstrating
the existence of wide genetic divergence at the phenotypic level. The major cluster-I included six genotypes,
cluster-IV and VI with three genotypes, cluster-II with two genotypes and other clusters with only solo genotype
based on similar morphological traits. Further, twenty ISSR primers were used to estimate the genetic diversity
and documentation of genotypes through molecular characterization. Thirteen ISSR primers were shown
polymorphism with 93 loci across the Gomphrena genotypes. Among these 93 loci, 79 were polymorphic (86.02
%) in nature, portraying the high allelic diversity. All the primers displayed good discriminating power, as
indicated by their PIC values, with a mean value of 0.21. Molecular profiling through MegaX and PCoA analyses
also separated the Gomphrena globosa population into two clusters and placed them into two plots.

How to Cite

Kumar, D. L., Kulkarni, B. S., P, P. K., & Chittapur, R. B. (2023). Morphological and molecular characterization of Gomphrena genotypes. Indian Journal of Horticulture, 80(04), 326–332. https://doi.org/10.58993/ijh/2023.80.4.4

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References

Ashwini, A., Kurubar, A. R., Patil, S. and Kumar, C. S. 2019. Evaluation of bachelor’s button (Gomphrena globosa L.) genotypes for growth and yield parameters. J. Pharmogn. Phytochem. 8(4): 541-545.

Baliyan, D., Sirohi, A., Kumar, M., Kumar, V., Malik, S. and Sharma, S. 2014. Comparative genetic diversity analysis in chrysanthemum: A pilot study based on morpho-agronomic traits and ISSR markers. Sci. Hort. 167: 164-168.

Cole, C. T. 2003. Genetic variation in rare and common plants. Annu. Rev. Ecol. Evol. Syst. 34: 213-237.

Doyle, J. J. and Doyle, J. L. 1990. Isolation of plant DNA from fresh tissue. Focus., 12:13-15.

Ghehsareh, M. G., Salehi, H., Khosh-Khui, M. and Niazi, A. 2015. Application of ISSR markers to analyze molecular relationships in Iranian jasmine (Jasminum spp.) accessions. Mol Biotechnol. 1:65-74.

Hale, A. L., Farnham, M. W. and Menz, M. A. 2006. Effectiveness of pcr-based markers for differentiating elite broccoli inbreds. J. Am. Soc. Hortic. Sci. 131: 418-423.

Herold, A., Lewis, D. H. and Walker, D. A. 1976. Sequestration of cytoplasmic orthophosphate by mannose and its differential effect on photosynthetic starch synthesis in C3 and C4 species. New Phytologist. 76(3): 397-407.

Mezghani, N., Debbabi, O. S., Rouz, S. and Medini, M. 2015. AFLP markers for the assessment of genetic variability in rose (Rosa gallica L.) cultivars in Tunisia. Inter. J. of Adv. Res., 3: 35-42.

Naik, H., B., Patil, A. A., Basavaraj, N. and Patil, V. S. 2005. Stability analysis for growth, yield and flower colour (xanthophyll) in African marigold (Tagetes erecta L.). Kar. J. Hort. 1(3): 28-36.

Namita, Panwar, S., Sonah, H., Singh, K. P. and Sharma, T. M., 2013, Genetic diversity analysis of marigold (Tagetes sp.) genotypes using RAPD and ISSR markers. Indian J. Agri. Sci., 83(5): 484-90.

Narsude, P. B., Kadam, A. S. and Patil, V.K. 2010. Studies on the growth and yield attributes of different African marigold genotypes under Marathwada conditions. The Asian J. Hort. 5(2): 284-286.

Niu, Y. F., C. M. Han, Shao, Y. and Tao, Y.D. 2009. Comparative study on eight trace elements in twelve flower medicines. Spectroscopy Spectral Anal. 29:1997-2000.

Panwar, S., Singh, K. P. and Namita. 2012. Assessment of variability, heritability components and grouping of Indian Rose (Rosa hybrida) genotypes based on DUS guidelines. Indian J. Agric. Sci. 82(10): 875-80.

Patil, V., Kulkarni, B. S., Reddy, B. S., Kerure, P. and Ingle, A. 2011. Yield and quality parameters as influenced by seasons and genotypes in marigold (Tagetes erecta L.). Res. J. Agric. Sci. 2(2): 344-347.

Roriz, C. L., Barros, L., Carvalho, A. M., Santos-Buelga, C., Ferreira and Isabel C. F. R. 2014. Pterospartum tridentatum, Gomphrena globosa and Cymbopogon citratus: A phytochemical study focused on antioxidant compounds. Food Res. Int. 62: 684-693.

Satya, P. 2012. Prezygotic interspecific hybridization barriers between kenaf (Hibiscus cannabinus L.) and four wild relatives. Plant Breeding. 131(5): 648-655.

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