Comparison of mango genomic DNA isolation methods for next generation sequencing

Abstract

Six different methods of DNA isolation namely, CTAB, CTAB with PVP, Qiagene DNA extraction Mini and Maxi kits, CTAB with addition of PVP and purification using PCI followed by Qiagen Genomic-tip 500/G were compared for recovery of quality DNA from mango leaves. The higher yield (1375.0 ng/ μl) of good quality (A260/280 and A260/230 1.80 & 1.90, respectively) DNA was obtained with modified CTAB method with addition of PVP (MW, 40,000) followed by purification using phenol: chloroform: isoamylalcohol 25:24:1 and Qiagen Genomic-tip 500/G as compared to standard CTAB method (1096.50 ng/ μl; A260/280 and A260/230 1.40 and & 1.10, respectively). The DNA obtained using modified CTAB method was found suitable for PCR, PacBio, ddRAD sequencing and long-term storage.