Optimization of enzymatic maceration for extraction of carotenoids and total phenolics from sweet pepper using response surface methodology
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Red capsicum, carotenoids, enzyme assisted extraction; response surface methodology; viscozymeIssue
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Abstract
Response Surface Methodology (RSM) is used in situations where several input variables potentially influence some performance measure or quality characteristic of a process. In this paper, a three-level, threefactor, Box-Behnken design under response surface methodology (RSM) was used to optimize the conditions of enzyme assisted processing of red capsicum juice. The effects of enzyme concentration (0.2-0.4%), incubation temperature (50-70°C) and time (30-90 min.) on juice yield, total soluble solids, total carotenoids and other bioactives were investigated. Overall, two to three-fold increase in total carotenoids and phenols was observed compared to control. From a response surface analysis of the data, a three-degree polynomial equation was developed which provided the following optimal extraction conditions: T = 50.98°C, extraction time = 32.08 min. and enzyme concentration = 0.351%. Under the optimal conditions, juice extracted from enzyme treated capsicum macerate (CM) had high total carotenoid content (50.43 mg/100 g) and juice yield (77.75%). Results demonstrate that viscozyme is a potential enzyme combination for enhancing recovery of total carotenoids and thereby increases the functional quality of the juice.
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