In vitro regeneration of tuberose through petals and immature flower buds
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Bulb, flower bud, in vitro regeneration, petal, tuberose.Issue
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Abstract
Unlike many other bulbous flower crops, where in vitro propagation techniques have been perfected, tuberose does not have enough background studies. A study was carried out to establish aseptic cultures and to standardize in vitro propagation protocol with petal segment and immature flower bud as explants. The explants pre-treated with carbendazim 0.1% + mancozeb 0.1% and 8-HQC (200 mg l-1) for 2½ h and surface sterilized with HgCl2 (0.1%) + NaOCl (1.0%) for 8 min., respectively were found to be the best in getting aseptic culture. The best medium for shoot multiplication was MS medium supplemented with 6.0 mg/l BAP + 0.5 mg/l NAA + 0.7 mg/l 2, 4-D + 0.5 mg/l TDZ for both petals (4.0) and immature flower bud (4.33) enhanced shoot multiplication. Irrespective of explants, maximum roots were observed on half-strength MS medium + 1.0 mg/l IBA. The in vitro regenerated micro-plant lets were successfully acclimatized in glass jar with polypropylene cap.
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