Regulation of cold stress and biochemical responses in marigold var. Pusa Narangi Gainda during the winter season

Published

2024-12-30

DOI:

https://doi.org/10.58993/ijh/2024.81.4.13

Keywords:

Tagetes erecta L., Cold stress, Low plastic tunnel, Chito Oligosaccharide, Salicylic Acid, Arbuscular mycorrhiza
Dimensions Badge

Issue

Vol. 81 No. 04 (2024): Indian Journal of Horticulture

Section

Research Articles

License

Copyright (c) 2024 Indian Journal of Horticulture

Creative Commons License

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Crossref
Scopus
Google Scholar
Europe PMC

Authors

  • Rayavarapu Tejaswi Division of Floriculture and Landscaping, ICAR-Indian Agricultural Research Institute, 110012, New Delhi, India
  • Prabhat Kumar Krishi Bhavan, Ministry of Agriculture and Farmers Welfare, New Delhi, 110012, India
  • K.P. Singh Division of Floriculture and Landscaping, ICAR-Indian Agricultural Research Institute, 110012, New Delhi, India
  • M.C. Singh Centre for Protected Cultivation Technology, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India
  • Sapna Panwar Division of Floriculture and Landscaping, ICAR-Indian Agricultural Research Institute, 110012, New Delhi, India
  • Ritu Jain Division of Floriculture and Landscaping, ICAR-Indian Agricultural Research Institute, 110012, New Delhi, India
  • Tejukumar B.K. Division of Floriculture and Landscaping, ICAR-Indian Agricultural Research Institute, 110012, New Delhi, India
  • Vinay Kumar L.N. ICAR-Indian Agricultural Statistics Research Institute, New Delhi, 110012, India

Abstract

This study aimed to compare various methods to alleviate cold stress and investigate biochemical responses in marigold var. Pusa Narangi Gainda. This study was conducted over two years (2021-2022 and 2022-2023) at the Centre for Protected Cultivation Technology (CPCT), ICAR-Indian Agricultural Research Institute, New Delhi involving the sowing of marigold variety Pusa Narangi Gainda on three different dates. The seedlings were then cultivated under two different growing conditions, using various treatments and their respective combinations. The biochemical responses were assessed and analysed. Results revealed that plants cultivated inside a low plastic tunnel showed the lowest activity of anti-oxidant enzymes, proline content, malondialdehyde content, total phenol content, total soluble sugars and soluble protein content significantly compared to those grown in open field conditions. Additionally, the plants from the sowing date of October 15th recorded the lowest values compared to the other two sowing dates (November 1st and November 15th). Through the examination of different treatments, it was observed that the combination of Chito Oligosaccharide at a concentration of 200 ppm with Arbuscular mycorrhiza recorded the increased anti-oxidant enzyme activity, proline content, total phenol content, total soluble sugars, soluble protein content and reduced malondialdehyde content comparing to the control and other treatments. This study showcases the beneficial effects of using low plastic tunnels in different sowing dates and treatments to increase plant resilience to cold stress.

How to Cite

Tejaswi, R., Kumar, P., Singh, K., Singh, M., Panwar, S., Jain, R., … Kumar L.N., V. (2024). Regulation of cold stress and biochemical responses in marigold var. Pusa Narangi Gainda during the winter season. Indian Journal of Horticulture, 81(04), 425–430. https://doi.org/10.58993/ijh/2024.81.4.13

Downloads

Download data is not yet available.

References

Aebi. H. 1984. Catalase in vitro. Methods Enzymol, 105:121-26.

Bates, L. S., Waldren, R. P. and Teare, I. D. 1973. Rapid determination of free proline for water-stress studies. Plant Soil, 39: 205–07.

Bradford, M. M. 1976. A Rapid and Sensitive Method for the Quantification of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding. Analytical Biochemistry, 72: 248-54.

Castillo F. J., Penel C. and Greppin, H. 1984. Peroxidase Release Induced by Ozone in Sedum album Leaves: Involvement of Ca. Plant Physiol. 74: 846-51.

Chen, S., Jin, W., Liu, A., Zhang, S., Liu, D., Wang, F., Lin, X. and He, C. 2013. Arbuscular mycorrhizal fungi (AMF) increase growth and secondary metabolism in cucumber subjected to low-temperature stress. Scientia Horticulturae, 160: 222–29.

Dhindsa, R. S., Dhindsa, P. P. and Thorpe, T. A. 1981. Leaf Senescence: Correlated with Increased Levels of Membrane Permeability and Lipid Peroxidation, and Decreased Levels of Superoxide Dismutase and Catalase. Journal of Experimental Botany, 32: 93-101.

Gill, T. 2010. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiology and Biochemistry, 48: 909-30.

Heath, R. L. and Packer, L. 1968. Photoperoxidation in isolated chloroplasts: I. kinetics and stoichiometry of fatty acid peroxidation. Archives of biochemistry and biophysics, 125: 189-98.

Homme, P. M., Gonzalez, B. and Billard, J. 1992. Carbohydrate Content, Frutane and Sucrose Enzyme Activities in Roots, Stubble and Leaves of Rye Grass (Lolium perenne L.) as Affected by Source/Link Modification after Cutting. Journal of Plant Physiology, 140: 282-91.

Kusvuran, S., Kiran, S. and Ellialtioglu, S. S. 2016. Antioxidant Enzyme Activities and Abiotic Stress Tolerance Relationship in Vegetable Crops. In Abiotic and Biotic Stress in Plants - Recent Advances and Future Perspectives, 490-92.

Li, Y., Zhang, Q., Ou, L., Ji, D., Liu, T., Lan, R., Li, X. and Jin, L. 2020. Response to the cold stress signaling of the tea Plant (Camellia sinensis) elicited by chitosan oligosaccharide. Agronomy.

Liu, A., Chen, S., Wang, M., Liu, D., Chang, R., Wang, Z., Lin, X., Bai, B. and Ahammed, G. J. 2015. Arbuscular mycorrhizal fungus alleviates chilling stress by boosting redox poise and antioxidant potential of tomato seedlings. Journal of Plant Growth Regulation.

Pasbani, B., Salimi, A., Aliasgharzad, N. and Hajiboland, R. 2020. Colonization with arbuscular mycorrhizal fungi mitigates cold stress through improvement of antioxidant defence and accumulation of protecting molecules in eggplants. Scientia Horticulturae, 272: 109575.

Shi, X., Sun, Z., Xue, X., Xu, H., Wu, Y., Zhang, Y., Yang, Y., Han, S., Zhao, R., Zhang, M. and Zhang, X. 2023. Amelioration of Hypothermia-Induced Damage on Peanut by Exogenous Application of Chitooligosaccharide. Agriculture, 13: 217.

Singleton, V. L. and Rossi, J. A. 1965. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture, 16: 144-58.

Tan, C., Li, N., Wang, Y., Yu, X., Yang, L., Cao, R. and Ye, X. 2023. Integrated Physiological and Transcriptomic Analyses Revealed Improved Cold Tolerance in Cucumber (Cucumis sativus L.) by Exogenous Chitosan Oligosaccharide. International Journal of Molecular Sciences, 24: 6202.

Wang, A., Li, J., AL-Huqail, A.A., AL-Harbi, M.S., Ali, E.F., Wang, J., Ding, Z., Rekaby, S.A., Ghoneim, A.M. and Eissa, M.A. 2021. Mechanisms of Chitosan Nanoparticles in the Regulation of Cold Stress Resistance in Banana Plants. Nanomaterials, 11: 2670.

Wu, Q. S. and Zou, Y. N. 2010. Beneficial roles of arbuscular mycorrhizas in citrus seedlings at temperature stress. Scientia Horticulturae, 125: 289-93.

Xie, W., Xu, P. and Liu, Q. 2001. Antioxidant activity of water-soluble chitosan derivatives. Bioorganic and Medicinal Chemistry Letters, 11: 1699–701.

Zhou, M., Chen, H., Wei, D., Ma, H. and Lin, J. 2017. Arabidopsis I3 and DELLAs positively regulate each other in response to low temperature. Sci. Rep. 7: 1–13.

Zou P, Tian X, Dong B and Zhang C. 2017. Size effects of chitooligomers with certain degrees of polymerization on the chilling tolerance of wheat seedlings. Carbohydrate Polymers, 160: 194-202.

Similar Articles

1 2 3 4 5 6 7 8 9 10 > >> 

You may also start an advanced similarity search for this article.

Most read articles by the same author(s)

1 2 3 > >>