In vitro organogenesis in Aloe barbadensis Mill.: An aloin A rich plant

Abstract

Efficient plant regeneration in Aloe barbadensis Mill. was achieved using callus derived from shoot meristem. Organogenesis was maximum on MS medium supplemented with 2.5 mg/l BA, 0.25 mg/l IAA. Though sucrose (2%) showed maximum shoot bud regeneration capacity, D-glucose had an intermediatory effect, however, fructose and maltose had no effect at all. Of the conditions tested shoot bud regeneration was highest (82.8) under 12 h illumination. The regenerative potential was stable up to 12 sub-cultures. The rate of shoot bud regeneration was dependent on the concentration of hormones in the nutrient media. Regenerated shoots rooted on half-strength MS medium containing 0.1 mg/l IAA and 1% sucrose. The in vitro derived plantlets were hardened in the nethouse with 75% light and successfully established in soil.