ISSR and RAPD marker based DNA fingerprinting and diversity assessment of Annona spp. in South Andamans

Abstract

Annona spp. is one of the most important wild fruit tree having great medicinal value. The present study was aimed to assess the inter-relationships among four Annona species collected from various parts of south Andaman ecosystem. A set of 30 ISSR and 20 RAPD primers were taken for DNA fingerprinting, among them 14 ISSR and 14 RAPD primers produced 31 and 48 amplicons out of which 18 and 25 amplicons were polymorphic having 58 and 52% polymorphism, respectively. The maximum discriminating band was obtained from primer ISSR 7 and ISSR 9. Cluster analysis of ISSR divided the four species into three clusters while with RAPD it was two clusters. An assessment of genetic diversity among four Annona species would assist in planning for future germplasm collection, conservation and domestication programmes.