Effect of genotype, explant and culture medium on organogenesis in brinjal

Published

2011-09-27

Keywords:

Egg plant, plant regeneration, organogenesis, shoot induction
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Authors

  • K.A. Mir Department of Vegetables Crops, Punjab Agricultural University, Ludhiana 141 004
  • A.S. Dhatt Department of Vegetables Crops, Punjab Agricultural University, Ludhiana 141 004
  • J.S. Sandhu Department of Vegetables Crops, Punjab Agricultural University, Ludhiana 141 004
  • A.S. Sidhu Department of Vegetables Crops, Punjab Agricultural University, Ludhiana 141 004

Abstract

Brinjal were regenerated from callus derived from hypocotyl, cotyledon and root explants of five genotypes, namely Punjab Barsati, Punjab Sadabahar, Jamuni Gola, PBSR-11 and BB-93C on MS medium containing different concentrations of IAA and BAP. A combination of 2.5 mg/l IAA + 0.5 mg/l BAP was found optimum for adventitious shoot induction from all explants. Genotype, explant and genotype x explant interaction showed highly significant effects on organogensis. Among genotypes, PBSR-11 showed maximum response for organogenesis (79.43%). However, among explants, cotyledon was significantly better than hypocotyl and root. Plants regenerated via adventitious shoots were rooted on half-strength MS basal medium in vitro.

How to Cite

Mir, K., Dhatt, A., Sandhu, J., & Sidhu, A. (2011). Effect of genotype, explant and culture medium on organogenesis in brinjal. Indian Journal of Horticulture, 68(03), 332–335. Retrieved from https://journal.iahs.org.in/index.php/ijh/article/view/1912

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