In vitro propagation of a self-incompatible cabbage line ‘Sel. 5’
Downloads
Published
Keywords:
Cabbage, self-incompatibility, micropropagation, growth hormones.Issue
Section
License
Copyright (c) 2013 Indian Journal of Horticulture
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Abstract
A viable protocol was developed for in vitro maintenance and multiplication of cabbage SI line ‘Selection-5’ with very strong S-allele interaction. Different types of explants, viz., apical bud, axillary bud and basal shoot sprout were tested for their in vitro regeneration ability. The morphogenetic potential varied among the explants. Apical bud proved to be the most potent explant for initial culture establishment followed by axillary bud. Murashige and Skoog (1962) medium supplemented with 2 mg l-1 BA, 0.5 mg l-1 NAA and 0.1 mg l-1 GA3 was optimum for culture establishment. The maximum in vitro shoot proliferation (5.89 ± 0.38) was obtained on MS medium supplemented with 5 mg l-1 KIN + 0.1 mg l-1 NAA + 0.1 mg l-1 GA3. The proliferation rate was significantly influenced by type and concentration of cytokinins with kinetin being more effective than BA. Half-strength MS medium supplemented with 1.0 mg l-1 IBA was most effective for rooting. The tissue cultured plants were successfully hardened and transferred to field conditions with a survival rate of 76.67 per cent.
How to Cite
Downloads
Similar Articles
- K.P. Sajith, S. Uma, M.S. Saraswathi, S. Backiyarani, P. Durai, Macropropagation of banana - Effect of bio-fertilizers and plant hormones , Indian Journal of Horticulture: Vol. 71 No. 03 (2014): Indian Journal of Horticulture
- Sahar Mirzaei, S.K. Singh, K.P. Singh, K.V. Prasad, S.S. Sindhu, In vitro micropropagation of miniature rose cv. Candy Sunblaze , Indian Journal of Horticulture: Vol. 67 No. Special Issue (2010): Indian Journal of Horticulture
- B.N. Patel, M.P. Solanki, S.R. Patel, J.R. Desai, Effect of bio-fertilizers growth, physiological parameters, yield and quality of brinjal cv. Surati Ravaiya , Indian Journal of Horticulture: Vol. 68 No. 03 (2011): Indian Journal of Horticulture
- S.K. Upadhyay, J. Badyal, R.P. Awasthi, Effect of pruning intensities on growth, nut quality, yield and leaf nutrient status of pecan , Indian Journal of Horticulture: Vol. 68 No. 02 (2011): Indian Journal of Horticulture
- M.C. Jain, L.K. Dashora, Effect of plant growth regulators on physico-chemical characters and yield of guava cv. Sardar under high density planting system , Indian Journal of Horticulture: Vol. 68 No. 02 (2011): Indian Journal of Horticulture
- Arun Kumar, N. Sharma, Growth, flowering and physiological responses of olive trees to growth retardants under rain-fed conditions of Himachal Pradesh , Indian Journal of Horticulture: Vol. 75 No. 03 (2018): Indian Journal of Horticulture
- P.R. Meghwal, Arvind Kumar, Suresh Kumar, Performance of cactus pear at two geographical locations in Indian arid zone , Indian Journal of Horticulture: Vol. 75 No. 01 (2018): Indian Journal of Horticulture
- Rajbir Singh, R.R. Sharma, D.B. Singh, Effect of vermicompost on plant growth, fruit yield and quality of strawberries in irrigated arid region of northern plains , Indian Journal of Horticulture: Vol. 67 No. 03 (2010): Indian Journal of Horticulture
- K Rajan, A. Abdul Haris, L.K Prasad, Shivani ., Efficacy of conventional, solid soluble and liquid fertilizers applied through drip-fertigation on tomato , Indian Journal of Horticulture: Vol. 71 No. 02 (2014): Indian Journal of Horticulture
- B.K. Singh, S.R. Sharma, B. Singh, Combining ability for antioxidants and economic traits in cabbage , Indian Journal of Horticulture: Vol. 68 No. 04 (2011): Indian Journal of Horticulture
<< < 1 2 3 4 5 6 7 8 9 10 > >>
You may also start an advanced similarity search for this article.
Most read articles by the same author(s)
- S.S. Dey, R. Bhatia Dey, Chander Parkash, Raj Kumar, Heterosis and combining ability analysis in snowball cauliflower using indigenously developed CMS lines , Indian Journal of Horticulture: Vol. 74 No. 03 (2017): Indian Journal of Horticulture
- Reeta Bhatia, S.S. Dey, Raj Kumar, Genetic divergence studies in tulip (Tulipa gesneriana L.) , Indian Journal of Horticulture: Vol. 74 No. 04 (2017): Indian Journal of Horticulture
- S.S Dey, Reeta Bhatia, Chander Parkash, Pritam Kalia, R.N Barwal, Evaluation of cauliflower (Brassica oleracea var. botrytis) CMS (Ogura) lines for agronomic and floral traits , Indian Journal of Horticulture: Vol. 71 No. 03 (2014): Indian Journal of Horticulture
- S.S. Dey, T.K. Behera, A.D. Munshi, S. Rakshit, R. Bhatia, Utility of gynoecious sex form in heterosis breeding of bitter gourd and genetics of associated vegetative and flowering traits , Indian Journal of Horticulture: Vol. 69 No. 04 (2012): Indian Journal of Horticulture
- S.S. Dey, T.K. Behera, A.D. Munshi, Anand Pal, Combining ability analysis in bitter gourd , Indian Journal of Horticulture: Vol. 67 No. 03 (2010): Indian Journal of Horticulture
- Chander Parkash, S.S. Dey, Reeta Bhatia, M.R. Dhiman, Indigenously developed SI and CMS lines in hybrid breeding of cabbage , Indian Journal of Horticulture: Vol. 72 No. 02 (2015): Indian Journal of Horticulture
- Chander Parkash, Inheritance of quantitative characters in knol khol , Indian Journal of Horticulture: Vol. 68 No. 02 (2011): Indian Journal of Horticulture