Micropropagation of sweet orange cv. Mosambi through shoot tips and nodal segments

Abstract

The present study was undertaken with an objective to develop a protocol for micropropagation of sweet orange cv. Mosambi through shoot tips and nodal segments. Shoot tips of 0.5-1.0 cm, nodal and internodal segments of 1.0-1.5 cm were collected and cultured in Murashige and Skoog (MS) medium. The treatment NaOCl (1%)7 min.+ HgCl2 (0.2%) 3 min. + KCl (1%) 1 min. was found to be the best sterilization treatment. BAP (2.0 mgl-1) + casein hydrolysate (200 mgl-1 ) was found to be the best treatment for establishment medium with respect to maximum sprouting, minimum days taken for sprouting with highest number of shoots/explant. BAP (2.0 mgl-1) + Kin (1.0 mgl-1) + NAA (0.1 mgl-1) was observed to be the best treatment for multiplication medium with maximum shoot length and highest number of leaves. IBA (2.0 mgl-1) + NAA (0.1 mgl-1) + activated charcoal (500 mgl-1) was found to be significantly superior over all other treatments with respect to maximum root initiation percentage, days to root initiation, highest number of roots and highest root length for shoot tips and nodal segments.