Standardization of embryo rescue technique for grape hybrids under sub-tropical conditions
Downloads
Published
Keywords:
Embryo rescue, grape, sub-tropical region.Issue
Section
License
Copyright (c) 2010 Indian Journal of Horticulture

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Abstract
The development of new high yielding cultivars of good quality with large berry size is the main objective of seedless grape breeding programme. Seedless grapes suffer from stenospermocarpy so, technique of embryo rescue employing in-ovulo culture has been adopted in grape breeding in western world which not only produce superior seedless cultivars but also curtails 7-8 years from entire breeding cycle. With respect to mean ovule age (days after pollination) and ovule-embryo growth (2.13 mm2), culturing of ovules 24 days after pollination was found to be the best. The concentration of IAA (4 mg/l) + GA3 (0.5 mg/l) proved to be most effective for germination (13.84 %). Among various media used for culturing, ovules on MS medium (1/2 macro + 1/1 micro) registered highest germination (13.75%). Chilling treatment was proved to be one of the important factors for embryo maturation and 60 days of chilling treatment at 40C was found better than 45 days. With respect to the effect of different growth regulators on various rooting and shooting parameters IBA (1.0 mg/l) and NAA (1.5 mg/l) were found superior. While comparing different hardening strategies, glass jar with polypropylene cap (GPP) was found to be most effective as far as hardening success (66.67%) was concerned.
How to Cite
Downloads
Similar Articles
- A.S Sundouri, Harminder Singh, M.I.S Gill, Anirudh Thakur, A.K Sangwan, In-vitro germination of hybrid embryo rescued from low chill peaches as affected by stratification period and embryo age , Indian Journal of Horticulture: Vol. 71 No. 02 (2014): Indian Journal of Horticulture
- Bhupendra Sagore, Kanhaiya Singh, Jai Prakash, Vartika Srivastava, Vignesh M, Brijesh Kumar Yadav, Elucidating the effect of plant bioregulators on embryo maturation for shortening the breeding cycle in papaya , Indian Journal of Horticulture: Vol. 80 No. 03 (2023): Indian Journal of Horticulture
- T. Boopathi, T. Manju, S.B. Singh, M. Arivalagan, S.K. Dutta, A.K.R. Singh, Y. Ramakrishna, Incidence of chewing pests in okra in north eastern hill region of India , Indian Journal of Horticulture: Vol. 72 No. 01 (2015): Indian Journal of Horticulture
- S.K. Dutta, Amrita Banerjee, R.S. Akoijam, Saurav Saha, Lungmuana ., Y. Ramakrishna, T. Boopathi, Somnath Roy, Vishambhar Dayal, Collection and phenotypic characterisation of pole-type common bean (Phaseolus vulgaris L.) landraces from Mizoram , Indian Journal of Horticulture: Vol. 75 No. 01 (2018): Indian Journal of Horticulture
- Sudip K. Dutta, R.S. Akoijam, T. Boopathi, S. Saha, S.B. Singh, S.K. Das, Ashish Yadav, Mango under high density planting: A case study from North East India , Indian Journal of Horticulture: Vol. 76 No. 02 (2019): Indian Journal of Horticulture
- T. Boopath, S.B. Singh, T. Manju, S.K. Dutta, A.R. Singh, Samik Chowdhury, Y. Ramakrishna, Vishambhar Dayal, Lungmuana ., Temporal modeling for forecasting of the incidence of litchi stink bug using ARIMAX analysis , Indian Journal of Horticulture: Vol. 74 No. 04 (2017): Indian Journal of Horticulture
- R.G. Somkuwar, Rutuja D. Nale, Pooja Karande, Ajay Kumar Sharma, P.H. Nikumbhe, Sharmistha Naik, Anju A. Bhagat, Deciphering the morphological variability in grape germplasm , Indian Journal of Horticulture: Vol. 81 No. 04 (2024): Indian Journal of Horticulture
- J.N. Kalbhor, Sharad Bhagwat, S.D. Shikhamany, Sumit, Rameshraddy, Variation in the relationship of bloom time petiole nutrient contents with yield in grape cv. Thompson Seedless and its clones , Indian Journal of Horticulture: Vol. 81 No. 01 (2024): Indian Journal of Horticulture
- J. K Soni, Vishambhar Dayal, S. K Sunani, Lalramhlimi Bawitlung, Amit Kumar, Ingudam Shakuntala, Sunil Doley, Improvement of ginger for yield and quality traits under the hill ecosystem of Mizoram , Indian Journal of Horticulture: Vol. 80 No. 03 (2023): Indian Journal of Horticulture
- Bikash Das, Sarita Mehta, S. K. Singh, Santosh Mali, M. K. Dhakar, A. K. Singh, Pruning effects on Sardar guava planted in ultra high density orcharding under different rainfall scenarios , Indian Journal of Horticulture: Vol. 75 No. 04 (2018): Indian Journal of Horticulture
You may also start an advanced similarity search for this article.
Most read articles by the same author(s)
- Nripendra V. Singh, S.K. Singh, V.B. Patel, In vitro culture establishment studies on pomegranate , Indian Journal of Horticulture: Vol. 68 No. 03 (2011): Indian Journal of Horticulture
- R.S. Pan, A.K. Singh, Stability of yield and its components in vegetable amaranth , Indian Journal of Horticulture: Vol. 75 No. 02 (2018): Indian Journal of Horticulture
- M. Tamil Selvan, Room Singh, S.K. Singh, Morphological characterization of newly evolved mango hybrids , Indian Journal of Horticulture: Vol. 67 No. Special Issue (2010): Indian Journal of Horticulture
- R.S. Pan, A.K. Singh, Stability of green pod yield and its components in faba bean (Vicia faba L.) , Indian Journal of Horticulture: Vol. 73 No. 03 (2016): Indian Journal of Horticulture
- A.K. Singh, Sanjay Singh, V.V. Appa Rao, B.G. Bagle, T.A. More, Efficacy of organic mulches on soil properties, earthworm population, growth and yield of aonla cv. NA7 in semi- arid ecosystem , Indian Journal of Horticulture: Vol. 67 No. Special Issue (2010): Indian Journal of Horticulture
- M. Tamil Selvan, Room Singh, S.K. Singh, Physico-chemical characters of some newly evolved mango hybrids , Indian Journal of Horticulture: Vol. 67 No. Special Issue (2010): Indian Journal of Horticulture
- D.K. Tiwari, V.B. Patel, Kalyan Barman, Ravindra Kumar, R.B. Verma, S.N. Singh, B.B. Mishra, R.G. Khandekar, Studies on influence of preharvest bagging of fruits on quality of mango cv. Ratna , Indian Journal of Horticulture: Vol. 76 No. 01 (2019): Indian Journal of Horticulture
- Prananath Barman, S.K. Singh, V.B. Patel, A.K. Singh, Lata Nain, Synergistic interaction of arbuscular mycorrhizal fungi and mycorrhiza helper bacteria improving antioxidant activities in Troyer citrange and Cleopatra mandarin under low moisture stress , Indian Journal of Horticulture: Vol. 72 No. 01 (2015): Indian Journal of Horticulture
- A. Nagaraja, K. Usha, Bhupinder Singh, S.K. Singh, C. Umamaheswari, Effect of temperature and relative humidity on growth and sporulation of Fusarium mangiferae under in vitro conditions , Indian Journal of Horticulture: Vol. 68 No. 01 (2011): Indian Journal of Horticulture
- J.K. Ranjan, S.K. Singh, A.K. Chakrabarti, Pragya ., In vitro shoot regeneration from cotyledonary leaf explant in chilli and bio-hardening of plantlets , Indian Journal of Horticulture: Vol. 67 No. 01 (2010): Indian Journal of Horticulture